African Journal of
Bacteriology Research

  • Abbreviation: J. Bacteriol. Res.
  • Language: English
  • ISSN: 2006-9871
  • DOI: 10.5897/JBR
  • Start Year: 2009
  • Published Articles: 103

Full Length Research Paper

Effect of high temperature on viability of Lactobacillus casei and analysis of secreted and GroEL proteins profiles

Najla Haddaji
  • Najla Haddaji
  • Laboratoire d?Analyse, Traitement et Valorisation des Polluants de l?Environnement et des Produits, Faculté de Pharmacie Rue Avicenne, Monastir 5000, Tunisie,
  • Google Scholar
Boubaker Krifi
  • Boubaker Krifi
  • Laboratoire d?Analyse, Traitement et Valorisation des Polluants de l?Environnement et des Produits, Faculté de Pharmacie Rue Avicenne, Monastir 5000, Tunisie,
  • Google Scholar
Rihab Lagha
  • Rihab Lagha
  • Laboratoire d?Analyse, Traitement et Valorisation des Polluants de l?Environnement et des Produits, Faculté de Pharmacie Rue Avicenne, Monastir 5000, Tunisie,
  • Google Scholar
Sadok Khouadja
  • Sadok Khouadja
  • Laboratoire d?Analyse, Traitement et Valorisation des Polluants de l?Environnement et des Produits, Faculté de Pharmacie Rue Avicenne, Monastir 5000, Tunisie,
  • Google Scholar
Amina Bakhrouf
  • Amina Bakhrouf
  • Laboratoire d?Analyse, Traitement et Valorisation des Polluants de l?Environnement et des Produits, Faculté de Pharmacie Rue Avicenne, Monastir 5000, Tunisie,
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  •  Received: 29 January 2015
  •  Accepted: 13 March 2015
  •  Published: 30 April 2015

Abstract

The bacterial heat shock response is characterized by the elevated expression of a number of chaperone complexes including the GroEL and the rate change of synthesis of certain proteins (total and secreted). In this work, after incubation at 45°C, total and secreted proteins profiles of stressed bacteria were found to be altered when analyzed by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE). In addition, the level expression of GroEL was evaluated with Western blot. Our results show a marked increase in both GroEL expression and protein synthesis at 45°C. These modifications were manifested by the appearance and/or disappearance of bands as well as in the level of expression of certain proteins.
 
Key words: Lactobacillus casei, heat shock, GroEL, secreted and total proteins, sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE), Western blotting.