Journal of
Cell and Animal Biology

  • Abbreviation: J. Cell Anim. Biol.
  • Language: English
  • ISSN: 1996-0867
  • DOI: 10.5897/JCAB
  • Start Year: 2007
  • Published Articles: 261

Full Length Research Paper

Cloning and expression of Theileria parva T-complex 1 protein zeta subunit ortholog

Nyerhovwo J. Tonukari1* and Richard T. Kangethe2
1Department of Biochemistry, Delta State University, PMB 1, Abraka, Nigeria. 2Department of Biochemistry, University of Nairobi, P.O. Box 30197, 00100, Nairobi, Kenya.
Email: [email protected]

  •  Accepted: 02 September 2009
  •  Published: 31 October 2009


East coast fever (ECF) is a severe lymphoproliferative disease of cattle caused by the intracellular protozoan Theileria parva from the family Apicomplexa. Gene homologs encoding antigens from other apicomplexan parasites constitute a source of vaccine candidate antigens. An ortholog of the zeta subunit of T-complex protein 1 (TCP-1) which plays a role in protein folding, assembly and transport was identified within the Theileria parva genome. The deduced amino acid sequence of the T. parva ortholog has a 55% identity and 77% similarity to the same protein in a number of eukaryotes such as Danio rerio (zebra fish) and man. The identity of this protein within its own family (Apicomplexa) is slightly higher. The T. parvaTCP-1 zeta subunit gene was cloned and expressed in Escherichia coli and the recombinant protein was characterized.


Key words: T-complex protein, Theileria parva, apicomplexa, antigen.