Journal of
Cell and Animal Biology

  • Abbreviation: J. Cell Anim. Biol.
  • Language: English
  • ISSN: 1996-0867
  • DOI: 10.5897/JCAB
  • Start Year: 2007
  • Published Articles: 261

Full Length Research Paper

Assessment of viability, chromatin structure stability, mitochondrial function and motility of stallion fresh sperm by using objective methodologies

Giannoccaro Alessandra1, Lacalandra Giovanni Michele2, Filannino Angela2, Pizzi Flavia3, Nicassio Michele2, Dell’Aquila Maria Elena2 and Minervini Fiorenza1*
1Institute of Sciences of Food Production (ISPA), National Research Council (CNR), Via G. Amendola 122/O, 70125, Bari, Italy. 2Department of Animal Production, University of Bari, Strada Provinciale Casamassima km 3, 70010, Valenzano, Bari, Italy. 3Institute of Agricultural Biology and Biotechnology (IBBA), National Research Council (CNR), Via Bassini 15, Milan, Italy
Email: [email protected]

  •  Accepted: 22 December 2009
  •  Published: 28 February 2010


Methodologies, such as flow cytometry and computer assisted sperm analysis (CASA), provide objective, reproducible, rapid and multi-parametric evaluation of semen quality. In this study, semen samples collected from six stallions were analysed for viability (by propidium iodide), chromatin stability by sperm chromatin structure assay (SCSA) and mitochondrial membrane potential by JC-1 using flow cytometry. Total and progressive motility, average path velocity (VAP), curvilinear velocity (VCL) and straight-line velocity (VSL) were determined by CASA system. The cytofluorimetric analysis provided results with low intra-assay variability respect to motility analysis by CASA system. The data on viability and mitochondrial assessment were rather uniform between stallions. The SCSA was able to distinguish potential fertility levels between stallions. In fact statistical differences were found between stallions especially for %-DFI and SD-DFI parameter. The %-DFI parameter was negatively correlated with VCL parameter. The higher repeatability of %-DFI parameter respect to those of other SCSA parameters confirms the importance of this parameter notoriously related to fertility. In conclusion, the simultaneous assessment of different functional sperm parameters, by flow cytometry and CASA, may be allow to obtain detailed and repeatable evaluations of sperm quality in the stallion, usually not considered in breeding selection programs.


Key words: Flow cytometry, stallion, sperm quality.