Bone morphogenetic proteins (BMPs), belonging to the transforming growth factor-beta family, are crucial factors in follicular growth and development in the mammalian ovary. In this study, we examined the effects of BMP2, 4, 6 and 7 on goat granulosa cells by in vitro culture. The results showed that BMP2, 4 and 7 increased follicle-stimulating hormone receptor (FSHR) level in goat granulosa cells whereas they decreased luteinizing hormone receptor (LHR) level in both transcript and protein levels; however, BMP6 upregulated LHR transcript and protein level in goat granulosa cells, whereas it had no effect on FSHR level. FSHR knockdown increased granulosa cell apoptosis. Our data provided the ï¬rst evidence that BMP2, 4, 6 and 7 may inhibit granulosa cell apoptosis through the regulation of FSHR and/or LHR level. These ï¬ndings provided new insight into the biological functions of BMP2, 4, 6 and 7 for follicular development in goat ovary.
Key words: Granulosa cell, bone morphogenetic proteins (BMPs), follicle-stimulating hormone receptor (FSHR), luteinizing hormone receptor (LHR)
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