Journal of
Clinical Medicine and Research

  • Abbreviation: J. Clin. Med. Res.
  • Language: English
  • ISSN: 2141-2235
  • DOI: 10.5897/JCMR
  • Start Year: 2009
  • Published Articles: 103

Full Length Research Paper

Collagen deposition and cellular viability among UVB irradiated human dermal fibroblasts treated by platelets

Yohanes Widodo Wirohadidjojo
  • Yohanes Widodo Wirohadidjojo
  • Department of Dermatology and Venereology, Faculty of Medicine, Gadjah Mada University, Jln.Farmako, Sekip-Utara, Yogyakarta, Indonesia.
  • Google Scholar
Niken Trisnowati
  • Niken Trisnowati
  • Department of Dermatology and Venereology, Faculty of Medicine, Gadjah Mada University, Jln.Farmako, Sekip-Utara, Yogyakarta, Indonesia.
  • Google Scholar
Arief Budiyanto
  • Arief Budiyanto
  • Department of Dermatology and Venereology, Faculty of Medicine, Gadjah Mada University, Jln.Farmako, Sekip-Utara, Yogyakarta, Indonesia.
  • Google Scholar


  •  Accepted: 29 December 2011
  •  Published: 28 February 2012

Abstract

Prolonged or repeated low energy ultraviolet B (UVB) irradiation that can inhibit fibroblast proliferation, collagen synthesis, and stimulate collagen degradation is basic concept of skin photo-aging.  Recently, a study showed that human skin fibroblasts treated with platelets resulted in a significant higher proliferation rates compared to placebo. The effect of platelets in photo aged human skin fibroblasts, however, is still unclear. To know the effect of platelets to UVB irradiated fibroblasts focusing on proliferation rate and extracellular collagen deposition, various platelet concentrations were given to UVB irradiated normal human dermal fibroblasts. Fibroblast viability rates were determined by MTT-assay, whereas collagen deposition levels were determined by insoluble collagen Sirius red-assay. Data were analyzed by Kruskal-Wallis H and post-hoc by Mann-Whitney U. p <0.05 is accepted for significance difference. A group treated by 1.25 normal platelet concentration showed an increase cellular viability rates and collagen deposition levelsignificantly (p<0.01).  As a conclusion, platelet in 1.25 normal platelet concentrations may be a promising modality for the treatment of photo-aging skin.

 

Key words: Platelet, ultraviolet B (UVB), fibroblast proliferation, collagen deposition.