Journal of
Cancer Research and Experimental Oncology

  • Abbreviation: J. Cancer Res. Exp. Oncol.
  • Language: English
  • ISSN: 2141-2243
  • DOI: 10.5897/JCREO
  • Start Year: 2009
  • Published Articles: 53

Full Length Research Paper

Epstein Barr virus latent membrane protein-1 (LMP-1) expression and angiogenesis in Indonesian nasopharyngeal carcinoma (NPC)

Mardiah Suci Hardianti
  • Mardiah Suci Hardianti
  • Division of Hematology- Medical Oncology, Department of Internal Medicine, Faculty of Medicine, Gadjah Mada University, Yogyakarta, Indonesia.
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Johan Kurnianda
  • Johan Kurnianda
  • Division of Hematology- Medical Oncology, Department of Internal Medicine, Faculty of Medicine, Gadjah Mada University, Yogyakarta, Indonesia.
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Harijadi
  • Harijadi
  • Department of Anatomy Pathology, Faculty of Medicine, Gadjah Mada University, Yogyakarta, Indonesia.
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Kartika Widayati Taroeno-Hariadi
  • Kartika Widayati Taroeno-Hariadi
  • Division of Hematology- Medical Oncology, Department of Internal Medicine, Faculty of Medicine, Gadjah Mada University, Yogyakarta, Indonesia.
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Rizka Humardewayanti Asdie
  • Rizka Humardewayanti Asdie
  • Division of Infectious Disease and Tropical Medicine, Department of Internal Medicine, Faculty of Medicine, Gadjah Mada University, Yogyakarta, Indonesia.
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Bambang Hariwiyanto
  • Bambang Hariwiyanto
  • Department of Ear-Nose and Throat, Faculty of Medicine, Gadjah Mada University, Yogyakarta, Indonesia.
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  • Article Number - E51D2F93527
  • Vol.2(4), pp. 47-53, November 2010
  •  Accepted: 19 October 2010
  •  Published: 30 November 2010

Abstract

The pivotal role of Epstein Barr virus latent membrane protein 1 (LMP-1) in angiogenesishas been concluded from several in vitro experiments. However, there were very limited and inconsistent data on the correlation between LMP-1 and angiogenesis from clinical samples. Therefore, we aimed to investigate the correlation between LMP-1 and angiogenesis from Indonesian nasopharyngeal carcinoma (NPC). Forty cases of paraffin embedded tissues were stained by immunohistochemistry for the expressions of LMP-1, proangiogenic factors including vascular endothelial growth factor (VEGF) and interleukin (IL)-8, and von Willebrand factor (vWF) indicating microvessels density (MVD) as the final result of angiogenesis. The level of plasma VEGF165 was determined by ELISA. The results showed that LMP-1 expression was neither correlated significantly with 10logMVD, 10log plasma and tissue VEGF, nor 10log IL-8 (r=0.273, p=0.088; r=-0.109, p=0.504; r=0.140, p=0.390 and r=0.274, p=0.087, respectively). However, there were trends that a higher expression of LMP-1 was followed by a higher expression of MVD, tissue VEGF and IL-8. In conclusion, our results may be supportive to the experimental findings on the role of LMP-1 in angiogenesis. Not less important, our study that also showed significant correlation between tissue VEGF and MVD and between IL-8 and MVD suggests a possible role for antiangiogenic therapy in nasopharyngeal carcinoma.

 

Key words: LMP-1, angiogenesis, NPC.