Journal of
Dentistry and Oral Hygiene

  • Abbreviation: J. Dent. Oral Hyg.
  • Language: English
  • ISSN: 2141-2472
  • DOI: 10.5897/JDOH
  • Start Year: 2009
  • Published Articles: 133

Full Length Research Paper

Gender determination using primary teeth: A polymerase chain reaction (PCR) study

Prashant M. Battepati1 and M. Shodan2*
1Department of Pedodontics and Preventive Dentistry, SDM College of Dental Sciences and Hospital, Sattur, Dharwad-580009, Karnataka, India. 2Department of Public Health Dentistry, SDM College of Dental Sciences and Hospital, Sattur, Dharwad-580009, Karnataka, India.
Email: [email protected]

  •  Accepted: 06 September 2013
  •  Published: 31 October 2013

Abstract

The aim of this study was to assess the effect of various environmental factors on the preservation of pulp tissue in primary teeth as a source of DNA and its use for sex determination using polymerase chain reaction (PCR). 120 samples were grouped into 4 groups of 30 teeth each. Group I samples were kept immersed in a bucket of drainage water, group II, III and IV samples were buried in sand taken from seashore, burial ground and desert, respectively for a period of 2 months. Pulp tissue was collected from each sample and DNA was isolated. PCR amplification was performed and sex determination was done by detection of X and Y chromosome-specific alphoid centromeric repeat sequences. In group I, 86% of the samples exhibited correct gender interpretation by PCR amplification. In groups II, III, and IV, all the samples showed correct results indicating a significant difference in scores between group I and the remaining three groups. Teeth stored in dry conditions can serve as a better source of DNA as compared to the teeth stored in moist conditions and co-amplification of both X and Y specific sequences by PCR is a fast, specific, sensitive and reliable method providing sex determination

 

Key words: DNA, gender, polymerase chain reaction (PCR), forensic