Journal of
Environmental Chemistry and Ecotoxicology

  • Abbreviation: J. Environ. Chem. Ecotoxicol.
  • Language: English
  • ISSN: 2141-226X
  • DOI: 10.5897/JECE
  • Start Year: 2009
  • Published Articles: 197

Full Length Research Paper

Polyamine levels and pigment contents in rapeseed regenerated in vitro in the presence of zinc

  Asma Ben Ghnaya1,2*, Annick Hourmant1,2,3, Michel Couderchet4, Michel Branchard1,2 and  Gilbert Charles1,2,5
  1Université Européenne de Bretagne, France. 2Laboratoire de Biotechnologie et Physiologie Végétales, ESMISAB, Université de Bretagne Occidentale Technopôle Brest-Iroise, 29280 Plouzané, France. 3Laboratoire de Toxicologie Alimentaire et Cellulaire, Université de Bretagne Occidentale, UFR Sciences et Techniques, C.S. 93 837, 29238 Brest Cedex 3, France. 4Laboratoire Plantes Pesticides et Développement Durable, Université de Reims Champagne-Ardenne, URVVC-SE, BP 1039, 51687 Reims, France. 5Laboratoire d’Ecophysiologie et Biotechnologie des Halophytes et Algues Marines (LEBHAM), Université de Bretagne Occidentale, IUEM, Technopole Brest Iroise, Place Nicolas Copernic, 29280 Plouzané, France.
Email: [email protected]

  •  Accepted: 28 April 2011
  •  Published: 31 August 2011



The present work was designed to select hyperaccumulator plants by in vitro breeding from transversal thin cell layers. The thin layer explants of Brassica napus cv. Jumbo were cultivated directly in the presence of ZnSO4 at different concentrations (0.1 to 1 mM). Regenerated shoots were transferred into pots, acclimatized, and cultured in the greenhouse. After 3 weeks, the regenerants were treated with 2 mM of ZnSO4 during 7 days. Growth parameters, guaiacol peroxidase activity (GPOX), malondialdehyde (MDA), polyamines (PAs) and pigment contents were evaluated. ZnSOapplied during the regeneration process influenced significantly both physiological and biochemical characteristics of regenerated plants. Particularly, an increase of Zn level in the tissue culture induced an increase in MDA content and GPOX activity in the leaves and a decrease in pigment contents. Exposure to 2 mM Zn induced 112% increase in free polyamines in the leaves and roots, while this increase was as high as 399% for plants regenerated in the presence of the metal.


Key words: Phytoremediation, hyperaccumulator, in vitro, polyamines, malondialdehyde, guaiacol peroxidase, zinc stress.