Journal of
Entomology and Nematology

  • Abbreviation: J. Entomol. Nematol.
  • Language: English
  • ISSN: 2006-9855
  • DOI: 10.5897/JEN
  • Start Year: 2009
  • Published Articles: 135

Full Length Research Paper

Interaction of extract of Solanum verbascifolium (Solanaceae) with Metarhizium anisopliae (Ascomycota: Hypocreales) on Rhipicephalus microplus mortality (Acari: Ixodidae)

Olivares-Pérez, Jazmín1, Fernández-Ruvalcaba, Manuel2, Lina-García, Laura3, Peña-Chora, Guadalupe4 and Hernández-Velázquez, V. Manuel3*
1Facultad de Estudios Superiores – Cuautitlán UNAM, México. 2Centro Nacional de Investigación Disciplinaria en Parasitología Veterinaria – INIFAP 3Centro de Investigación en Biotecnología, Universidad Autónoma del Estado de Morelos (UAEM) Av. Universidad 1001, Col. Chamilpa, Cuernavaca, Morelos, C. P 62210 México. 4Centro de Investigaciones Biológicas, UAEM, México.
Email: [email protected]

  •  Accepted: 05 June 2013
  •  Published: 31 July 2013



The objective of this study was to evaluate the interaction of a plant extract (Solanum verbascifolium) and a strain of Metarhizium anisopliae selected for its virulence towardsRhipicephalus microplus. The treatments used were 1000, 10 000, 20 000 and 30 000 ppm dilutions of an aqueous extract of S. verbascifolium plus M. anisopliae (1 x 108 con/ml). S. verbascifolium, 20 000 ppm and M. anisopliae in an emulsion formulation provided an excellent alternative to the application of fungal conidia alone, and it could be effective to combine these substances with other control agents; however, more research is needed in this area. It is also advisable to assess the effect of the plant extract on the growth and sporulation of the fungus and determine the potentially protective effect of the oil emulsion on the fungal conidia.


Key words: Plant extract, entomopathogenic fungus, tick.