Abstract

This study discusses and developed methods for obtaining plant extracts/pure compounds and its usages as a nematicidal agent. Freshly hatched second-stage juveniles of two nematode species, Meloidogyne incognita and Cephalobus litoralis were used. A bioassay guided isolation of the extract, fractions and pure compounds were done for their nematicidal activity at different concentrations in comparison with Azadirachta indica, while distilled water was taken as control. The crude extract showed 90% and ethyl acetate fraction 97% mortality rate after 48 h at 1% concentration against M. incognita species and 81 and 50% against C. litoralis specie at the same concentration. Among the pure compounds, 4 and 9 showed maximum mortality of 90 and 91% and compounds 8, 3, 6, 2, 5, 7 and 1 showed 89, 88, 88, 82, 80, 80 and 69% mortality, respectively after 48 h in M. incognita sp. In C. litoralis, compounds 8 and 9 showed 72 and 75%, significant mortality, while 7, 4, 3, 5, 6, 2 and 1 showed 70, 70, 70, 68, 62, 60 and 58% mortality, respectively after 48 h. The plant is of economic importance with nematicidal value.

Key words: Meloidogyne incognita, Cephalobus litoralis, compound, mortality, crude extract.