Journal of
Entomology and Nematology

  • Abbreviation: J. Entomol. Nematol.
  • Language: English
  • ISSN: 2006-9855
  • DOI: 10.5897/JEN
  • Start Year: 2009
  • Published Articles: 130

Full Length Research Paper

Effect of different concentrations of Eriobotrya japonica extract on control of infection by Meloidogyne incognita and Cephalobus litoralis

Nighat Sultana*
  • Nighat Sultana*
  • Pharmaceutical Research Centre, PCSIR Laboratories Complex, Karachi-75280, Pakistan.
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Musarrat Akhtar
  • Musarrat Akhtar
  • Pharmaceutical Research Centre, PCSIR Laboratories Complex, Karachi-75280, Pakistan.
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Sadia Ferheen
  • Sadia Ferheen
  • Pharmaceutical Research Centre, PCSIR Laboratories Complex, Karachi-75280, Pakistan.
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Razia Sultana Bina
  • Razia Sultana Bina
  • G.C University Lahore, Pakistan; Biotechnology Research Centre, PCSIR Laboratories Complex, Karachi-75280, Pakistan
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Ghafoor Ahmed
  • Ghafoor Ahmed
  • HEJ Research Institute of Chemistry, International Centre for Chemical and Biological Sciences, University of Karachi, Karachi-75270, Pakistan
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  •  Accepted: 06 January 2014
  •  Published: 28 February 2014

Abstract

This study discusses and developed methods for obtaining plant extracts/pure compounds and its usages as a nematicidal agent. Freshly hatched second-stage juveniles of two nematode species, Meloidogyne incognita and Cephalobus litoralis were used. A bioassay guided isolation of the extract, fractions and pure compounds were done for their nematicidal activity at different concentrations in comparison with Azadirachta indica, while distilled water was taken as control. The crude extract showed 90% and ethyl acetate fraction 97% mortality rate after 48 h at 1% concentration against M. incognita species and 81 and 50% against C. litoralis specie at the same concentration. Among the pure compounds, 4 and 9 showed maximum mortality of 90 and 91% and compounds 8, 3, 6, 2, 5, 7 and 1 showed 89, 88, 88, 82, 80, 80 and 69% mortality, respectively after 48 h in M. incognita sp. In C. litoralis, compounds 8 and 9 showed 72 and 75%, significant mortality, while 7, 4, 3, 5, 6, 2 and 1 showed 70, 70, 70, 68, 62, 60 and 58% mortality, respectively after 48 h. The plant is of economic importance with nematicidal value.

Key words: Meloidogyne incognita, Cephalobus litoralis, compound, mortality, crude extract.