Lupines turmos plants had been used by some Sudanese patients with type 2 diabetes, and Glibenclamide, an anti-diabetic drug had been the commonly used one. This study deals with the determination of the biological effects of L. turmos, used traditionally by Sudanese patients in comparing the anti-diabetic drug (Glibenclamide). This study focuses on type 2 diabetic patients who use some plants as treatment without any experimental data on the biological effects, suitable dose and role in diabetic treatment. Some patients with type 2 diabetes suffer some side effects of the drug. The assay of the present study was conducted on albino rats, obtained from the Faculty of Pharmacy, University of Khartoum, Sudan. 39 rats of either sex, weighing (135 to 250 g) and aged two months old were used. The animals were divided randomly into five groups (N=6). Group 1 served as control, Group 2 was treated with antidiabetic drug (Glibenclamide 10 mg/kg bodyweight (b.w)) and Groups 3, 4 and 5 are administered with three different doses (200, 400 and 800 mg/kg b.w) L. turmos aqueous extract, respectively. Blood specimens were collected from each group and serum levels of blood glucose, lipid profile and Î±-amylase concentrations were estimated. The results shows an insignificant difference between the means of blood glucose in two treated groups; Group 4 treated with (400 mg/kg b.w) L. turmos aqueous extract (blood glucose 111.9 mg/dl) and Group 2 treated with (10 mg/kg b.w) Glibenclamide (blood glucose 98.416 mg/dl). Cholesterol and triglycerides of treated groups were the same as in Group 3 with (10 mg/kg b.w) Glibenclamide; also, there was no significant difference between the two treated groups and control group. Aqueous extract of the plant inhibited Î±-amylase enzyme activity at dose (200 mg/kg b.w) in Group 3 versus Group 2 with Glibenclamide drug and there was no significant difference between the two groups (pâ‰¤0.05). It can be concluded that L. turmos aqueous extract have a hypoglycemic effect by reducing both blood glucose and Î±-amylase enzyme without any side effect.
Keywords: Î±-Amylase, cholesterol, diabetes mellitus, glibenclamide, insulin, L. turmos, pancreas and triglycerides.