Journal of
Horticulture and Forestry

  • Abbreviation: J. Hortic. For.
  • Language: English
  • ISSN: 2006-9782
  • DOI: 10.5897/JHF
  • Start Year: 2009
  • Published Articles: 273

Full Length Research Paper

In vitro propagation of white-flower mutant of Rhynchostylis gigantea (Lindl.) Ridl. through immature seed-derived protocorm-like bodies

Zhi-Ying Li* and Li Xu
Institute of Tropical Crop Genetic Resources, Chinese Academy of Tropical Agricultural Sciences / Key Laboratory of Tropical Crops Germplasm Resources Utilization, Ministry of Agriculture, Hainan Danzhou 571737 , P. R. China.
Email: [email protected]

  • Article Number - 6E58CB540082
  • Vol.1(6), pp. 093-097, August 2009
  •  Accepted: 20 July 2009
  •  Published: 31 August 2009

Abstract

Rapid in vitro propagation of white-flower mutant of Rhynchostylis gigantea (Lindl.) Ridl. was established for the first time through 4 month old immature seed-derived protocorm-like bodies (PLBs). 75% of PLBs developed from immature seeds cultured on half-strength MS medium with 0.05 mg·l-1 6-BA and 0.2 mg·l-1 NAA within 70 days upon transfer to MS medium with 2.0 mg/l-1 BA and 1.0 mg·l-1 NAA were induced to produce callus. Maintenance of the callus was achieved by subculturing the callus at an interval of 2 months on medium having the same level of growth regulators. The callus turned up to protocorm-like bodies upon transfer to hormone-free MS medium after 30 days of culture and later developed to shoots with 2-4 leaves. Transfer of the emerged shoots to half strength MS medium containing 100 g·l-1 banana homogenate facilitated root development and shoot growth. Plantlets established ex vitro exhibited 95% survival.

 

Key words: Plant regeneration, protocorm, Rhynchostylis gigantea (Lindl.) Ridl., somatic embryogenesis.

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