Journal of
Horticulture and Forestry

  • Abbreviation: J. Hortic. For.
  • Language: English
  • ISSN: 2006-9782
  • DOI: 10.5897/JHF
  • Start Year: 2009
  • Published Articles: 286

Full Length Research Paper

Effects of mycorrhiza, organo-mineral and NKP fertilizer on the performance and post harvest quality of sweetcorn

Etukudo O.O
  • Etukudo O.O
  • Department of Agronomy, University of Ibadan, Ibadan, Oyo State, Nigeria.
  • Google Scholar
Babatola L. A.
  • Babatola L. A.
  • Department of Agronomy, University of Ibadan, Ibadan, Oyo State, Nigeria.
  • Google Scholar
Ojo O. D
  • Ojo O. D
  • National Horticultural Research Institute, Ibadan, Oyo State, Nigeria.
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Fagbola O.
  • Fagbola O.
  • Department of Agronomy, University of Ibadan, Ibadan, Oyo State, Nigeria.
  • Google Scholar

  •  Received: 21 February 2015
  •  Accepted: 11 March 2015
  •  Published: 01 April 2015


A field experiment was carried out to assess the effects of vesicular arbuscular mycorrhiza (VAM), Organo-mineral fertilizer (OMF) and NPK (15:15:15), on the performance and post-harvest quality of sweet corn. The experiment was laid out in a split plot design with mycorrhiza as main plot and fertilizer combinations as treatments. VAM inocula were applied at a rate of 888.88 kg ha-1, OMF at 2000 kg ha-1 and NPK (15:15:15) at 400kg ha-1. Storability of the sweet corn was evaluated at open shelf (25-27°C), normal refrigeration (40°C) and deep freezing conditions (0°C). Results showed that there were significant differences (p <0.05) between mycorrhiza and non mycorrhiza. Across mycorrhiza treatment, the highest yields of free corn ears were obtained with the complementary use of OMF and NPK (15:15:15). Fertilizer application had significant effect on the storage life of sweet corn, however, sweet corn stored by freezing at 0°C had the best storage properties in terms of firmness, weight loss and disease incidence.


Key words: Vesicular arbuscular mycorrhiza; sweetcorn yield; Organo-mineral fertilizer.


Sweet corn (Zea Mays, L.) is gradually becoming an important vegetable crop in Nigeria, since it forms a useful ingredient in the preparation of salad and other food both at home and in hotels (Akintoye and Olaniyan, 2012). It is one of the few vegetables that originated in North America. Canned sweetcorn in salads is popular in Europe. Sweet corn powder can be use as thickening agents for soup and noodles. It has a lower calorie and higher protein concentration compared to the field corn; it is also a good source of dietary fiber.
In Nigeria, sweetcorn production and utilizations have not been a viable option to farmers despite the numerous benefits and economic importance. This is due to the decline in soil fertility, which prevents optimum yield, and the short shelf life of sweet corn. Akintoye and Olaniyan (2012) noted sweet corn as a heavy feeder requiring high amount of macro nutrients. With management practices such as continuous cropping and reduced fallow periods, tropical soils can hardly support cropping without supplementations (CIMMYT 1989/90).
Although there are environmental criticisms towards the use of inorganic fertilizer (Stone et al., 1995; Dormaar and Chang, 1995; Akintoye and Olaniyan, 2012), its use is still a must (Hera, 1996), since the land is limited and the demand for higher production is pressing. Despite the global campaign towards organic fertilizers, it must be realized that their cost and other constraints frequently deter farmers from using them in recommended quantities and in balanced proportions. Hence, a judicious combination of mineral fertilizers with organic sources of plant nutrient is being promoted. In the same vein, through biological based technology, crop production can be economical and sustainable with emphases on enhanced production, through increased resource productivity on farms with limited resources in which mycorrhiza comes to play.
Food storage at all levels of production is essential since no producer will increase his production if he has no way of storing the excesses. A huge economic loss to farmers results from post-harvest produce wastages as a result of lack or insufficient storage facility. There has been a consensus by authors regarding deteriorations and post-harvest loss of fruits and vegetables in all countries (Cappellini and Ceponis, 1984; Harvey, 1978), thus, the aim of good storage facility is to reduce this waste to the barest minimum. Brecht (2004) stated that storing sweet corn at 30°C for 24 h reduced the sugar content by 60%. Although there is limited information on the post-harvest quality of sweetcorn in Nigeria, several storage techniques such as perforated package (Riad and Brecht, 2002), shrink-wrapping (Aharoni et al., 1996), modified atmosphere (Morales-Castro et al., 1994) and controlled freezing-point (Shao and Li, 2011) have been critically investigated elsewhere.
Consequently, this study was designed to evaluate the effects of mycorrhiza, organo-mineral fertilizer (OMF) and inorganic fertilizer on the performance and storability of sweetcorn under an open shelf, normal and freezer conditions. 


Pre-cropping soil sampling
Before ploughing, soil sampling was done at the depth of 15 cm and 30 cm which was assumed to be the active rooting zone for corn. Sampling was done based on the history of the land. Composite samples were taken from the plots. These were bulked and air-dried in the shade for 7 days after which the samples were grounded and thoroughly mixed. They were then sieved using 2 mm and 0.5 mm sieves. These prepared samples were analyzed for physical and chemical properties at the analytical laboratory of the International Institute for Tropical Agriculture (IITA), Ibadan, Nigeria.
Experimental design
A   field  experiment  was  conducted  in  the  National   Horticultural Research Institute (NIHORT), Ibadan Nigeria (7° 541 N; 7° 301 E), to determine the effects of mycorrhiza, pacesetter OMF (Grade A) and golden fertilizer NPK (15:15:15) on the performance and storability of sweet corn. The vesicular arbuscular mycorrhiza (VAM) was obtained from IITA, Ibadan. The variety of sweet corn used was T21035 (Zea mays L.) The corn was sourced from the NIHORT, Ibadan. The experiment was laid out on split plot design with a 2 x 2 x 2 factorial arrangement. Mycorrhiza inoculation formed the main plot treatment, the application of OMF and NPK (15:15:15) were sub-plot treatments with three replicates. Plot size measured 3 m x 2 m (6 m2) and separated by 2 m alleyways between the main plots and 1 m alleyways between the sub-plots.  The experimental plot was harrowed twice before planting. Plant spacing of 75 cm x 30 cm was used to give a plant population of 44, 444 plants ha-1.  20 g of VAM fungal inocula were placed few centimeters below the sweet corn seedling root in each VAM fungus treatment, making up an application rate of 888.88 kg ha-1. OMF at the rate of 2000 kg ha-1 and NPK (15:15:15) at the rate of 400 kg ha-1 were applied simul-taneously in each fertilizer treatment. Fertilizer application was done in two splits, at 14 and 42 days after planting (DAP). Weeding was done manually at 20 and 35 DAP, subsequent weed emergences were hand pulled to ensure a weed free field.
Growth and yield assessment
Measurements on plant height, stem girth, number of leaves and leaf area were taken weekly. Leaf area was calculated according to the model given by Saxena and Singh (1965) as:
0.75 × Length × Width
Harvesting of sweet corn was done at 98 DAP when the cobs were still green.
Storage experiment
Harvested sweetcorn was stored under three storage conditions viz: ambient condition (25-27°C, RH 83%), refrigeration (4°C, RH 87%) and deep freezing (0°C, 90%). Data were collected on storage weight loss.
Loss in fresh weight (%) = Difference in weight/    Initial weight    ×    100
Firmness and decay or mould growth were subjectively scored. Firmness was determined by hand-feel and rated on a scale, where 1= firm, 2=moderately firm, 3=moderately soft and 4= soft. Mould growth on cobs with grains were observed and rated as 1= intact, 2= slight attack, 3= high incidence, 4=unmarketable.
Data analysis
Analysis of variance procedures were performed for all data to test treatments effects on the various parameters measured. Significant means (p< 0.05) were separated using Duncan’s Multiple Range test (Duncan, 1955). Data were analyzed using Statistical Analysis System software, version 8.0 (SAS, 1999).


Field experiment
The soil was found to be sandy loamy on textural triangle and slightly acidic (pH  6.0)  (Table  1).  Total  N  value  of 1.08%, was less than the critical level of 1.15% (Enwezor et al., 1989) and the phosphorus level was found to be lower than 10-16 mg kg-1 critical level reported by Adeoye and Agboola (1985). The 0.11 cmol kg-1 obtained in this study for potassium was less than the critical level of 0.18-0.20 cmol kg-1 (Agboola and Obigbesan, 1975). This result suggests that the soil used for the study was very low in major nutrient elements and would respond favourably to fertilizer application. 
The effect of mycorrhiza on the growth parameter is presented in Tables 2 and 3. At 2 WAP, there was sig-nificant effect of mycorrhiza inoculation only on stem girth (p<0.05). Leaf area and stem girth were significantly dif-ferent between the inoculated and non-inoculated plants at 4, 6 and 8 WAP with mycorrhiza inoculated sweetcorn having the highest significant means throughout. Although mycorrhiza inoculation significantly affected all growth parameters, the time of expression varies. As the mycorrhiza effects manifestation started as early as 2 WAP on the stem girth, number of leaves did not increase significantly until 8WAP. The reason for this variation is not far from the difference in the physiological requirements of different plant parts. The reliability of VAM to enhance the P uptake of the crop has been reported to vary with length of fallow, tillage practices, soil nutrient status, fertilizer and host crop (Thompson, 1994). This was confirmed in the present study as leaf area showed appreciable increase with mycorrhiza and fertilizer treatment combinations over the plant without mycorrhiza and fertilizer. The interactive effect of mycorrhiza and fertilizer combinations on the yield of sweet corn is presented in Table 4. 
Irrespective of the type of fertilizer used, sweet corn with mycorrhiza generally had more yield than their counterparts with no mycorrhiza. The significant increase in sweet corn yield for mycorrhiza-inoculated plants underscores the positive effects of VAM in plant nutrition. The reliant of plant nutrition, especially phosphorus uptake, on VAM have been documented (Howler et al., 1987; Osonubi et al., 1991; Atayese et al., 1993; Fagbola et al., 1998). Tas (2014) also reported a  higher  yield  for sweet corn inoculated with mycorrhiza. With mycorrhiza inoculation, combination of NPK with OMF gave the highest significant yield. This result is in variance with the report of Akintoye and Olaniyan (2012) who obtained higher yield from plot with NPK relative to organic fertilizer and organo-mineral fertilizer, similar report was also documented by Paul and Beauchamp (1993). How-ever, the better yield under the complementary use of OMF and NPK has been reported by several authors (Titiloye, 1982; Akpomudjere and Omueti, 1991; Sodunke, 1997). 
Storage experiment
The three storage methods showed significant differences (P <0.05) for the three post harvest indices (Table 5). While storage in deep freezer was best across the three indices used, storage under ambient condition was the worst. The result showed that loss in quality of sweet corn was greatly slowed down by storage under cold freezer conditions. Better storability of sweet corn in deep freezer  than  in  the  normal  refrigerator  and  open shelf as indicated by firmness, percentage weight loss and percentage disease incidence agrees with the report of Willis et al. (1981). The authors reported that tem-perature and humidity were the most important factors in the shelf life of fruit and vegetables. Also, the observed reduction in spoilage of sweetcorn due to refrigeration was in line with the report of Babatola and Olaniyi (1998). 


Mycorrihiza inoculation and the complementary use of organo-mineral and inorganic fertilizer are very essential for enhancing soil nutrient status and increasing crop yield. Storage of sweetcorn is very important for prospects in its production, as corn stored in deep freezer was better than those in the normal refrigerator and open shelf in terms of firmness, percentage weight loss and disease incidence.


Adeoye GO, Agboola AA, (1985). Critical levels for soil pH, available P,K,Zn and maize leaf content of P, Cu and Mn in sedimentary soils of Southwestern Nigeria. Fertilizer Resour. 6(1): 65
Agboola AA, Obigbesan GO (1975). The responses of some improved crop varieties to fertilizer in forest zone of Eastern Nigeria. Report of FAO/Red. Department of Agriculture, Seminar on use of fertilizer in Nigeria.
Aharoni Y, Copel A, Gil M, Fallik E (1996). Polyolefin stretch films maintain the quality of sweet corn during storage and shelf-life. Postharvest Biol. Technol. 7(1-2):171-176.
Akintoye HA, Olaniyan AB (2012). Yield of sweet corn in response to fertilizer sources. Glob. Adv. Res. J. Agric. Sci. 1(5):110-116.
Akpomudjere E, Omueti JAI (1991). Effect of NPK fertilizer and FYM on growth and yield of upland rice (Oryza sativa L). Proceedings of the 8th Anuual Conference of the Soil Science Society of Nigeria, Ile-Ife.
Atayese MO, Awotoye OO, Osonubi O, Mulongoy K (1993). Comparison of influence of Vesicular Arbuscular Mycorrhiza on the productivity of hedge row woody legumes and cassava at the top and the base of a hill slope under alley cropping system. Biol. Fertility Soil 16:198-204.
Babatola LA, Olaniyi JO (1998). Growth and shelf life of Amaranthus caudatus under different levels of NPK fertilization and storage conditions. Proceedings of the 16th HORSON conference, UNAAB, Abeokuta, Nigeria. pp. 273-277.
Brecht JK (2004). Postharvest quality maintenance guidelines. In: Gross K, Wang CY, Saltveit M, editors. The Commercial Storage of Fruits, Vegetables, and Florist & Nursery Crops. 3rd ed. Washington, D.C., USA.
Cappellini, RA, Ceponis, MJ (1984). Postharvest losses in fresh fruits and vegetables. p. 24-30. In: Moline HE (ed.), Postharvest pathology of fruits and vegetables: postharvest losses in perishable crops. Univ. Calif. Bull. 1914.
CIMMYT (1989/90). World Maize Facts and Trends: Realizing the Potential of Maize in Sub-Saharan Africa. v, 71 pags. Mexico, DF (Mexico). CIMMYT. Series: CIMMYT World Maize Facts and Trends.
Dormaar JF, Chang C (1995). Effect of twenty annual applications of excess feedlot manure on labile soil phosphorus. Canad. J. Soil Sci. 75:507-512.
Duncan DB (1955). Multiple range and multiple F-test. Biometrics. 11:1-42.
Enwezor WO, Udo EJ, Usoroh NJ, Ayotade KA, Adepetu JA, Chude VO, Udegbe CI (1989). Fertilizer use and management for crops in Nigeria. Series N0 2. P 163.
Fagbola O, Osonubi O, Mulongoy K (1998). Contribution of Arbuscular Mycorrhiza (AM) fungi and hedge row trees to the yield and nutrient uptake of cassava in an alley cropping system. J. Agric. Sci. 131:79-85.
Harvey JM (1978). Reduction of losses in fresh market fruits and vegetables. Annu. Rev. Phytopathol. 16:321-341.
Hera C (1996). Role of inorganic fertilizer and their management practices. Khiwer Academic Publishers. Fertilizer Resour. 43:63-81.
Howler RH, Sieverding E, Saif S (1987). Practical aspect of Mycorrhizal technology in some tropical crops and pastures. Plant Soil. 100:249-283.
Morales-Castro J, Rao MA, Hotchkiss JH, Downing DL (1994). Modified atmosphere packaging of sweet corn on cob. J. Food Process Preserv. 18(4): 279-293.
Osonubi O, Mulongoy K, Okali DUU (1991). Effects of ectomycorrhiza fungi on drought tolerance of four leguminous woody seedlings. Plant Soil 136:131-143.
Paul JW, Beauchamp EG (1993). Nitrogen availability for corn in soils amended with urea, cattle slurry and solid and composted manures. Can. J. Soil Sci. 73:253-266.
Riad GS, Brecht JK (2002). Perforation-mediated modified atmosphere packaging of sweetcorn. Proc. Florida State Hort. Soc. 115:71-75.
SAS Institute (1999). SAS Users Guide Statistics SAS Institute. NC, USA.
Saxena MC, Singh Y (1965). A note on leaf area estimation of intact maize leaves. India J. Agron. 10:437-439.
Shao X, Li Y (2011). Quality control of fresh sweet corn in controlled freezing-point storage. Afr. J. Biotechnol. 10(65), pp. 14534-14542.
Sodunke MO (1997). Effects of combination of Organic materials with SRP on dry matter yield and nutrient uptake of maize. M.SC. Project report, University of Ibadan (Unpublished).
Stone KC, Huny PG, Coffey SW, Mathany TA (1995). Water quality status of USDA water quality demonstration project in the eastern coastal plain. J. Water Conserv. 50:567-570.
Tas B (2014). Effect of the Mycorrhiza Application on the Agronomical Properties of Sweet Corn Varieties. J. Agric. Allied Sci. 3(2):41-47.
Titiloye EO (1982). The evaluation of different types of organic waste as a source of nutrient for the growth and yield of maize. Ph.D Thesis. University of Ibadan.
Willis RBH, McGlasson WB, Graham D, Lee HT, Hall EG (1981). Postharvest: An introduction to the physiology and handling of fruits and vegetables. BSP Professional books, Oxford. pp 39-45.