Journal of
Microbiology and Antimicrobials

  • Abbreviation: J. Microbiol. Antimicrob.
  • Language: English
  • ISSN: 2141-2308
  • DOI: 10.5897/JMA
  • Start Year: 2009
  • Published Articles: 168

Full Length Research Paper

Detection of blaCTX-M, blaTEM, and blaSHV genes in ESBL-producing enterobacterales from poultry farms in the peri-urban area of Ouagadougou, Burkina Faso

Isidore Juste Ouindgueta Bonkoungou
  • Isidore Juste Ouindgueta Bonkoungou
  • Laboratory of Molecular Biology, Epidemiology and Surveillance of Bacteria and Viruses Transmitted by Food (LaBESTA), Joseph KI-ZERBO University, Ouagadougou 03 BP 7021, Burkina Faso.
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Marguerite Edith Malatala Nikiema
  • Marguerite Edith Malatala Nikiema
  • Laboratory of Molecular Biology, Epidemiology and Surveillance of Bacteria and Viruses Transmitted by Food (LaBESTA), Joseph KI-ZERBO University, Ouagadougou 03 BP 7021, Burkina Faso.
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Zakaria Garba
  • Zakaria Garba
  • Nanoro Clinical Research Unit (URCN), Health Sciences Research Institute (IRSS)/CNRST, Ouagadougou 11 BP 218, Burkina Faso.
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Evariste Bako
  • Evariste Bako
  • Laboratory of Molecular Biology, Epidemiology and Surveillance of Bacteria and Viruses Transmitted by Food (LaBESTA), Joseph KI-ZERBO University, Ouagadougou 03 BP 7021, Burkina Faso.
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Souleymane Belem
  • Souleymane Belem
  • Laboratory of Molecular Biology, Epidemiology and Surveillance of Bacteria and Viruses Transmitted by Food (LaBESTA), Joseph KI-ZERBO University, Ouagadougou 03 BP 7021, Burkina Faso.
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Djifahamaï Soma
  • Djifahamaï Soma
  • Laboratory of Molecular Biology, Epidemiology and Surveillance of Bacteria and Viruses Transmitted by Food (LaBESTA), Joseph KI-ZERBO University, Ouagadougou 03 BP 7021, Burkina Faso.
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Fatimata Bintou Josiane Diarra
  • Fatimata Bintou Josiane Diarra
  • Laboratory of Molecular Biology, Epidemiology and Surveillance of Bacteria and Viruses Transmitted by Food (LaBESTA), Joseph KI-ZERBO University, Ouagadougou 03 BP 7021, Burkina Faso.
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Barthélémy Sibiri Zoma
  • Barthélémy Sibiri Zoma
  • Laboratory of Molecular Biology, Epidemiology and Surveillance of Bacteria and Viruses Transmitted by Food (LaBESTA), Joseph KI-ZERBO University, Ouagadougou 03 BP 7021, Burkina Faso.
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Modeste Gampene
  • Modeste Gampene
  • Laboratory of Molecular Biology, Epidemiology and Surveillance of Bacteria and Viruses Transmitted by Food (LaBESTA), Joseph KI-ZERBO University, Ouagadougou 03 BP 7021, Burkina Faso.
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Namwin Siourimè Somda
  • Namwin Siourimè Somda
  • Food Technology Department (DTA), Research Institute of Applied Sciences and Technologies, CNRST, Ouagadougou 03 BP 7047, Burkina Faso.
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Souleymane Sore
  • Souleymane Sore
  • Ministry of Health, Ouagadougou 03 BP 7022, Burkina Faso.
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Nicolas Barro
  • Nicolas Barro
  • Laboratory of Molecular Biology, Epidemiology and Surveillance of Bacteria and Viruses Transmitted by Food (LaBESTA), Joseph KI-ZERBO University, Ouagadougou 03 BP 7021, Burkina Faso.
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  •  Received: 14 December 2024
  •  Accepted: 06 February 2025
  •  Published: 14 February 2025

Abstract

This study investigated the carriage of blaCTX-M, blaTEM and blaSHV gene in Extended-spectrum beta-lactamase (ESBL)-producing Enterobacterals from poultry in peri-urban area of Ouagadougou, Burkina Faso. From June to November 2023, fresh fecal samples were collected from 37 consenting poultry farms in four peri-urban areas around Ouagadougou. These samples were cultured to isolate ESBL-producing Escherichia coli and Klebsiella species. The isolates were then examined for the presence of blaCTX-M, blaTEM, and blaSHV genes and their antibiotic susceptibility patterns. Out of the 37 poultry samples, 27 ESBL-producing bacteria were recovered; 48.64% (18/37) were ESBL-producing E. coli (16 blaCTX-M genes, 8 blaTEM genes, 2 blaSHV genes) and 27.02% (10/37) were Klebsiella spp. (10 blaCTX-M genes, 8 blaSHV genes, 5 blaTEM genes). Co-carriage of blaCTX-M/TEM, blaCTX-M/SHV, and blaTEM/SHV, high resistance rates to tetracycline and trimethoprim-sulfamethoxazole, and multi-drug resistance (MDR) phenotypes were observed among the ESBL-producing bacteria isolated in this study. The findings indicate the widespread presence of ESBL-producing bacteria in poultry farms in Burkina Faso, highlighting a potential public health risk.
 
Key words: Detection of extended-spectrum beta-lactamase (ESBL), enterobacterales, poultry frams, peri-urban, Burkina Faso.

Copyright © 2025 Author(s) retain the copyright of this article.

This article is published under the terms of the Creative Commons Attribution License 4.0

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