Journal of
Microbiology and Antimicrobials

  • Abbreviation: J. Microbiol. Antimicrob.
  • Language: English
  • ISSN: 2141-2308
  • DOI: 10.5897/JMA
  • Start Year: 2009
  • Published Articles: 166

Full Length Research Paper

Phytochemical and antimicrobial screening of methanol extract of Heliotropium indicum leaf

  Osungunna, M. O.1* and Adedeji, K. A.2
        1Department of Pharmaceutics, Faculty of Pharmacy, Obafemi Awolowo University, Ile-Ife, Nigeria. 2Department of Pharmaceutical Microbiology, Faculty of Pharmacy, Olabisi Onabanjo University, Sagamu, Ogun State, Nigeria.
Email: [email protected]

  •  Accepted: 24 May 2011
  •  Published: 30 August 2011

Abstract

 

Methanol extract of the leaf of Heliotropium indicum was evaluated for its antimicrobial activity against five bacterial isolates comprising of four Gram-negative namely: Escherichia coli, Pseudomonas aeruginosa, Klebsiella spp. and Proteus mirabilis; and one Gram positive, Staphylococcus aureus at 6.25, 12.5, 25, 50, 100 and 200 mg/ml including phytochemical analysis. While both S. aureus and Klebsiella spp. were inhibited at 50, 100 and 200 mg/ml with minimum inhibitory concentration (MIC) of 3 mg/ml; P. aeruginosa and P. mirabilis with MIC of 10 mg/ml were inhibited at 100 mg/ml and 200 mg/ml. E. coli with MIC of 20 mg/ml was inhibited only at 200 mg/ml concentration of the extract. Phytochemical analysis revealed the presence of plant metabolites as alkaloids, saponin and tannins. While activity-directed assay is advocated, the plant promises to be of tremendous assistance in the treatment of infections with which bacterial isolates used for the study are associated. 

 

Key words: Phytochemical, antimicrobial, Heliotropium indicum alkaloids, saponin, tannin.