Journal of
Microbiology and Antimicrobials

  • Abbreviation: J. Microbiol. Antimicrob.
  • Language: English
  • ISSN: 2141-2308
  • DOI: 10.5897/JMA
  • Start Year: 2009
  • Published Articles: 166

Full Length Research Paper

Bacillus licheniformis IITRHR2: A novel source of antimicrobial proteinaceous food substance

Sapna Sharma1, Ram L. Singh2 and Poonam Kakkar1*
1Herbal Research Section, Indian Institute of Toxicology Research (CSIR) 80, M. G. Marg, Lucknow-226001, Uttar Pradesh, India. 2Department of Biochemistry, Dr. Ram Manohar Lohia Avadh University, Faizabad-244001, Uttar Pradesh, India.
Email: [email protected]

  •  Accepted: 13 November 2010
  •  Published: 30 December 2010


Spoilage of food due to contamination by bacteria present in the environment is a major problem which affects human health and efforts to identify antibacterial components from natural sources has gained momentum. In this regard we isolated a new strain of Bacillus licheniformis IITRHR2 (accession number FJ447354) which produced bacteriocin like inhibitory substance (BLIS). Partially purified BLIS showed a major peak on HPLC with antimicrobial activity. This proteinaceous substance (~1.2 kDa) was thermo-stable and pH resistant but lost activity when subjected to proteinase treatment. BLIS inhibited growth of 16 bacteria including environmental and clinical contaminants of food products and potentiated the activity of nisin. In conclusion, the proteinaceous substance isolated from B. licheniformisIITRHR2 showed potential to combat contamination of food products by bacteria present in the environment.


Key words:  Antimicrobial activity, Bacillus licheniformis IITRHR2, BLIS, nisin.