Journal of
Microbiology and Antimicrobials

  • Abbreviation: J. Microbiol. Antimicrob.
  • Language: English
  • ISSN: 2141-2308
  • DOI: 10.5897/JMA
  • Start Year: 2009
  • Published Articles: 157

Full Length Research Paper

The use of Lycopersicum esculentum Mill. leaves extract against the survival of fungi

Irene Ahou Kouadio*
  • Irene Ahou Kouadio*
  • Laboratory of Biochemistry and Food Sciences, UFR Biosciences, University of Felix Houphouet-Boigny, 22 BP 582 Abidjan 22, Ivory Coast, Cote d’Ivoire.
  • Google Scholar
Mathias Kouame Koffi
  • Mathias Kouame Koffi
  • Laboratory of Biochemistry and Food Sciences, UFR Biosciences, University of Felix Houphouet-Boigny, 22 BP 582 Abidjan 22, Ivory Coast, Cote d’Ivoire.
  • Google Scholar
Olivier Kouame Chatigre
  • Olivier Kouame Chatigre
  • Laboratory of Biochemistry and Food Sciences, UFR Biosciences, University of Felix Houphouet-Boigny, 22 BP 582 Abidjan 22, Ivory Coast, Cote d’Ivoire.
  • Google Scholar
Mireille Bretin Dosso
  • Mireille Bretin Dosso
  • Pasteur Institute of Ivory Coast 01 BP 490 Abidjan 01, Cote d’Ivoire.
  • Google Scholar


  •  Accepted: 06 December 2013
  •  Published: 31 January 2014

Abstract

In this study, the antifungal fraction of Lycopersicum esculentum Mill. leaves extract was evaluated for its effect against biomass and cell viability of three strains of Aspergillus (Aspergillus fumigatus, Aspergillus flavus and Aspergillus nidulans). The results obtained show a reduction of the RNA concentration representing the biomass with the increase of the antifungal fraction content in the medium. Indeed, the mean of the RNA concentrations in the medium without antifungal fraction were about 113, 83 and 69 times those observed in the medium at 1% of antifungal fraction, respectively for A. nidulans, A. fumigatus and A. flavus. This reduction of the biomass was related to a reduction of the surviving cells with the increase of the antifungal fraction content in the medium. Indeed, the percentage in reduction of Alamar blue indicating cell viability which was 100% in the medium without antifungal fraction for the three strains tested decreased to reach the values of 2.40, 2.67 and 3.52% in the medium at 1%, respectively, for A. nidulans, A. fumigatus and A. flavus after five days of incubation. Thus, L. esculentum leaves extract exhibit a real inhibitory effect against fungi with an ability of killing them.

Key words: Lycopersicum esculentum, antifungal fraction, Aspergillus, biomass, cell viability.