Journal of
Microbiology and Antimicrobials

  • Abbreviation: J. Microbiol. Antimicrob.
  • Language: English
  • ISSN: 2141-2308
  • DOI: 10.5897/JMA
  • Start Year: 2009
  • Published Articles: 160

Full Length Research Paper

Serotypes and antibiotypes of Salmonella isolated at the University Teaching Hospital of Yopougon, Abidjan, Cote d’Ivoire from 2005 to 2009

Boni-Cissé C.1*, Meité S.1, Faye-Ketté H.1, , Houedanou C.1, Timité-Konan M.2, Kalpi C.3,Bakayoko S.3, Nguessend N.3, Akessé N.3, Soumahoro K.3, and Dosso M.1,3
1Mother and Child Bacterial Unit of the Central Laboratory of the University Teaching Hospital of Yopougon, Abidjan, Côte d'Ivoire. 2Medical Pediatric Service University Teaching Hospital Yopougon, Côte d'Ivoire. 3National Reference Center of Salmonella of the Institute Pasteur of Cote d’Ivoire, Cote d’Ivoire
Email: [email protected]

  •  Accepted: 09 November 2011
  •  Published: 28 February 2012

Abstract

Salmonella are responsive of gastroenteritis or bacteremia through fecal-oral transmission.Salmonella are also incriminated in meningitis particularly in patients with immune deficiency. The emergence of new serotypes and antibiotic resistance in Salmonella is a public health concern in developing countries. The aim of this study is to determine Salmonella serotypes circulated in the country and describe antibiotic resistance phenotypes. A retrospective study was conducted from January 2005 to December 2009. The strains were identified according to standard bacteriological methods. Serotypes were determined by slide agglutination using sera according to Kauffmann-White scheme. Antimicrobial susceptibility was performed using Kirby- Bauer method and interpretation was made according to the Comité de l'Antibiogramme–Société Française de Microbiologi (CA-SFM). A total of 62 Salmonella strains were isolated from 2005 to 2009. These strains were identified in 53.2% of cases in blood cultures 16.1 % in stool and 3.2% in CSF. Typing was possible for 76.1% of strains. Among serotypes, non-typhoid Salmonella were prevalent, 76.1 versus 23.9% for Salmonella typhi. S. typhimuriumwas recorvered in 37% and Salmonella enteritidis 16%. The resistance rates ranged from 66.6 to 100% for amoxicillin, 50 to 75% for cotrimoxazole and 41.7 to 75% for chloramphenicol. Multidrugs resistance (to three or more antibiotics) rate was 34.1% for non-typhoid and 33.3% for S. typhi. Resistance to nalidixic acid was 38% and reduced susceptibility to ciprofloxacin 14%. This study highlight emergence of multidrug-resistant Salmonella to antibiotics in pediatric. Salmonella surveillance must be put in place in Côte d'Ivoire to improve epidemiological analysis of strains diversity.

 

Key words: Salmonella, pediatrics, serotype, antibiotic resistance