Journal of
Microbiology and Antimicrobials

  • Abbreviation: J. Microbiol. Antimicrob.
  • Language: English
  • ISSN: 2141-2308
  • DOI: 10.5897/JMA
  • Start Year: 2009
  • Published Articles: 157

Full Length Research Paper

Antimicrobial susceptibility and sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) typing of Gram negative bacteria isolated from urinary tract infections in Mansoura, Egypt

Abdelaziz Elgaml*
  • Abdelaziz Elgaml*
  • Microbiology and Immunology Department, Faculty of Pharmacy, Mansoura University, Egypt.
  • Google Scholar
Ramadan Hassan
  • Ramadan Hassan
  • Microbiology and Immunology Department, Faculty of Pharmacy, Mansoura University, Egypt.
  • Google Scholar
Rasha Barwa
  • Rasha Barwa
  • Microbiology and Immunology Department, Faculty of Pharmacy, Mansoura University, Egypt.
  • Google Scholar
Shadi Shokralla
  • Shadi Shokralla
  • Microbiology and Immunology Department, Faculty of Pharmacy, Mansoura University, Egypt. Biodiversity Institute of Ontario, University of Guelph, Canada.
  • Google Scholar
Wael El-Naggar
  • Wael El-Naggar
  • Microbiology and Immunology Department, Faculty of Pharmacy, Mansoura University, Egypt.
  • Google Scholar


  •  Accepted: 06 February 2014
  •  Published: 28 February 2014

Abstract

The aim of the study was to determine the resistance patterns of Gram negative bacterial isolates recovered from patients suffering from urinary tract infections (UTIs) in Mansoura university hospitals, Egypt and also to investigate their epidemiological relatedness using sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) technique. The most prevalent etiological agents of UTIs were the Gram-negative bacilli bacteria including Escherichia coli, Pseudomonas spp. and Klebsiella spp. Among the isolates, Pseudomonas spp. showed the highest antimicrobial resistance rate and was significantly resistant to most of the antimicrobials more than other isolates. Antimicrobial susceptibility testing showed that imipenem could be considered as the drug of choice for the treatment of infections caused by multi-resistant isolates of UTIs. SDS-PAGE classified the E .coli, Pseudomonas and Klebsiella isolates into 5, 2 and 5 types, respectively.

Key words: Gram negative bacteria, urinary tract infections (UTIs), antimicrobial susceptibility testing, sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE).