Journal of
Microbiology and Antimicrobials

  • Abbreviation: J. Microbiol. Antimicrob.
  • Language: English
  • ISSN: 2141-2308
  • DOI: 10.5897/JMA
  • Start Year: 2009
  • Published Articles: 166

Full Length Research Paper

Biological control of black pod disease of cocoa (Theobroma cacao L.) with Bacillus amyloliquefaciens, Aspergillus sp. and Penicillium sp. in vitro and in the field

Stephen Larbi-Koranteng
  • Stephen Larbi-Koranteng
  • Department of Crop and Soil Sciences, Faculty of Agriculture Education, University of Education, Winneba (UEW), College of Agriculture Education, Mampong Ashanti, Ghana.
  • Google Scholar
Richard Tuyee Awuah
  • Richard Tuyee Awuah
  • Department of Crop and Soil Sciences, Faculty of Agriculture, Kwame Nkrumah University of Science and Technology (KNUST), Kumasi, Ghana.
  • Google Scholar
Fredrick Kankam
  • Fredrick Kankam
  • Department of Agronomy, Faculty of Agriculture, University for Development Studies (UDS), Tamale, Ghana.
  • Google Scholar

  •  Received: 25 June 2020
  •  Accepted: 10 August 2020
  •  Published: 31 August 2020


Adebola MO, Amadi JE (2011). Studies on Penicillium digitatum, Botryodiploidia theobromae, Alternaria tenuis and Trichoderma harzianum for bicontrol of Phytophthora palmivora cocoa black pod disease pathogen. American-European Journal of Agronomy 5(2):30-34.


Adebola MO, Amadi JE (2012). The Efficacy of Paecilomyces sp. and Penicillium digitatum on black pod disease pathogen in the field. European Journal of Applied Science 4(3):101-104.


Adedeji AR, Odebode AC, Agbeniyi SO (2005). Biocontrol potential of mycoflora associated with cocoa pod, leaf, root and rhizosphere soil in Nigeria. Malaysian International Cocoa Conference, 18th-19th July 2005, Kuala Lumpur.


Adedeji AR, Odebode AC, Sanusi RA, Olaiyu OA (2010). Comparative efficacy and Economic viability of Trichoderma strains as bio-control agents for the control of Phytophthora pod rot of cocoa in Nigeria. African Research Review 4(3a):349-366.


Adejumo TO (2005). Crop protection strategies for major diseases of cocoa, coffee and cashew in Nigeria. African Journal of Biotechnology 4(2):143-150.


Agrios GN (2005). Plant Pathology (Fifth edition). Academic Press, London P 952.


Akrasi KO (2005). Anti-microbial properties of yam rhizophere microorganisms and their potential use for controlling yam tuber rot. MSc. Thesis. Faculty of Agriculture, KNUST, Kumasi. Ghana, P. 104.


Anderson LM, Stockwell VO, Loper JE (2004). An extracellular protease of Pseudomonas fluorescens inactivates antibiotics of Pontoea agglomerans. Phytopathologia 94:1228-1234.


Asiamah RD (1998). Soil and soil suitability of Ashanti Region. Soil Research Institute- Council for Scientific and Industrial Research, Kwadaso-Kumasi: Report No 193, p. 21.


Awuah RT, Frimpong M (2002). Cocoa-based media for culturing Phytophthora palmivora (Butl.) Butl., causal agent of black pod disease of cocoa. Mycopatologia 155:143-147.


Awuah RT, Akrasi KO (2012). Tuber rot of yam in Ghana and evaluation of some yam rhizosphere bacteria for fungitoxicity to yam rot fungi. Inter. Journal of AgriScience 2(7):571-582.


Beneduzi A, Ambrosini A, Passaglia LMP (2012). Plant growth-promoting rhizobacteria (PGPR): their potential as antagonists and biocontrol agents. Genetic and Molecular Biology 35:1044-1051.


Bhavani R, Abraham K (2005). Efficacy of selected epiphytic microflora from pod surface against Phytophthora pod rot of cocoa. In: Proceedings of national symposium on biotechnological interventions for improvement of horticultural crops. Issues and Strategies, Kerela Agricultural University, Kerala. pp. 398-400.


Campbell RB (1998). Biological control of microbial plant pathogens rot of citrus fruit by Debaryomyces hansenii. Plant Disease 74:134-137.


Chen XH, Koumoutsi A, Scholz R, Schneider K, Vater J, Sussmuth R, Piel J, Borriss R (2009). Genome analysis of Bacillus amyloliquefaciens FZB42 reveals its potential for biocontrol of plant pathogens. Journal of Biotechnology 140:27-37.


Choudhary DK, Johri BN (2009). Interactions of Bacillus spp. and plant with special reference to induced systemic resistance (IRS). Microbiology Research 164:493-513.


Commission Regulation (EC) (2006). No 1881/2006 of 19th December. Setting maximum levels of certain contaminants in foodstuff. OJL. 364.20.12. 2016, P5.


Darmano TW (1994). Capability of different isolates of Trichoderma spp. in suppressing Phytophthora spp. inoculum in cocoa in cocoa pod tissue. Menera Perkerbunan 62(2):25-29.


European Food Safety Authority (EFSA) (2009). Scientific Opinion of the Panel on contamination in the food chain on a request from the European Commission on cadmium in food. The EFSA Journal 980:1-139.


European Food Safety Authority (EFSA) (2012). Cadmium dietry exposure in European population. The European Food Safety Authority Journal 10(1):2551. (32 p)


Guetsky R, Shtienberg D, Elad Y, Fisher E, Dinoor A (2002). Improving biological control by combining biocontrol agents each with several mechanisms of disease suppression. Phytopathology 92:976-985.


Haas D, Keel C (2003). Regulation of antibiotic production in root-colonizing Pseudomonas spp. and relevance for biological control of plant disease. Annual Review of Phytopathology 41:117-153.


Janisiewicz JWJ (1998). Biocontrol of postharvest disease of applies with antagonistic mixtures. Phytopathology 78:194-198.


Jetiyanon K, Kloepper JW (2002). Mixtures of plant growth-promoting rhizobacteria for induction of systermic resistance against multiple plant diseases. Biological Control 24:285-291.


Kamilova F, Kravchenko LV, Shaposhnikov AL, Makarova N, Lugtenberg B (2006). Effects of the tomato pathogen Fusarium oxysporium f. sp. radicis-lycopersici and the bioncontrol bacterium Pseudomonas fluorescens WCS365 on the composition of organic acids and sugar in tomato root exudates. Molecular Plant-Microbe Interaction 19(10):1121-1126.


Koranteng SL, Awuah RT (2011). Biological suppression of black pod lesion development on detached cocoa pods. African Journal of Agriculture Research 6(1):67-72.


Loper JE, Buyer JS (1991). Siderophores in microbial interactions of plant surfaces. Molecular Plant-Microbe Interaction 4:5-13.


Lucy M, Reed E, Glick BR (2004). Application of free living plant growth-promoting rhizobacteria. Antonie Van Leeuwenhoek 86:A1-25.


Lugtenberg BJ, Kamilova F (2009). Plant-Growth-Promoting Rhizobacteria. Annual Review of Microbiology 63:541-556.


Melnick RL, Solis KS, Suárez C, Backman PA (2008). Field evaluation of endophytic endospore-forming bacteria with cocoa successions for management of witches' broom of cocoa during the dry season, 2007. Plant Disease Management Report 2:136.


Meteorological Service Department (2012). Mampong Municipal Assembly, Ghana-West Africa.


Milgroom MG, Cortesi P (2004). Biological control of chestnut blight with hypovirulence: a critical analysis. Annual Review of Phytopathology 42:311-338.


Niazi A, Manzoor S, Asari S, Bejai S, Meijer J, Bongcam-Rudloff E (2014). Genome Analysis of Bacillus amyloliquefaciens Subsp. plantarum UCMB5113: A rhizobacterium that improves plant growth and stress management. PLoS ONE 9 (8):e104651.


Odigie EE, Ikotun T (1982). In-vitro and in-vivo inhibition of growth of Phytophthora palmivora (Butl.) Butl. by antagonistic microorganisms. Fitopatologia Brasileira 7:157-167.


Ongena M, Jacques P, Troure Y, Destain J, Jabrane A, Thonart P (2005). Involvement of fengycin-type lipopeptide in the multifaceted biocontrol of Bacillus subtilis. Applied Microbiology and Biotechnology 69(1):29-38.


Raaijmakers JM, Vlami M, de Souza JT (2002). Antibiotic production by bacterial biocontrol agents. Antonie van Leeuwenkoek 81:537-547.


Raupach GS, Kloepper JW (1998). Mixtures of plant growth promoting rhizobacteria enhance biological control of multiple Cucumber pathogens. Phytopathology 88:1158-1164.


Robert RC (1990). Postharvest biological control of Apple decay by Crotoccocus laurentii. Phytopathology 80:526-530.


Shahraki MA, Heydari A, Hassanzadeh N (2009). Investigation of antibiotic, siderophore and volatile metabolites production by Bacillus and Pseudomonas bacteria. Iran Journal of Biology 22:71-85.


Somers E, Vanderleyden J, Srininasan M (2004). Rhizosphere bacterial signaling: a love parade beneath our feet. Critical Review of Microbiology 30:205-240.


Sutikno AL (1997). Cocoa pod for feeding of Ruminant livestock. Wartaz 6:38-43.


Vallad GE, Goodman RM (2004). Systemic acquired resistance and induced systemic resistance in conventional agriculture. Crop Science 44:1920-1934.


Walker TS, Bais HP, Grotewold E, Vivanco JM (2003). Root exudation and Rhizosphere Biology. Plant Physiology 132:44-51.


Weller DM (2007). Pseudomonas, biocontrol agent of soil borne pathogens: Looking back over 30 years. Phytopathology 97(2):250-256.


Whipps JM (2001). Microbial interactions and biocontrol in the rhizosphere. Journal of Experimental Botany 52:487-511.