July 2014

Purification and characterization of a bacteriocin produced by Lactococcus lactis subsp. lactis PD6.9

In this study, a bacteriocin produced by Lactococcus lactis subsp. lactis PD6.9 was purified, characterized and identified. The bacteriocin was purified to homogeneity from culture supernatant by cation exchange and reversed-phase liquid chromatography, and its molecular weight was determined by mass spectrometry. The presence of the nisin gene was confirmed by polymerase chain reaction (PCR) and DNA sequencing. The...

Author(s): Margarete Alice Fontes Saraiva, Ingolf Figved Nes, Maria Cristina Baracat-Pereira, Marisa Vieira de Queiroz, Hilário Cuquetto Mantovani and Célia Alencar de Moraes

July 2014

Evaluation of antibacterial effects and phytochemical screening of the aqueous and methanolic extracts of Hibiscus diversifolius

Hibiscus diversifolius which is widely distributed in Kenya was investigated for its antibacterial effect against Escherichia coli, Pseudomonas aeruginosa, Bacillus subtilis and Staphylococcus aureus. The leaves stem and root of the plant was extracted using aqueous and methanol as solvents. Phytochemical screening was also carried out to determine the phytochemical constituents present in the various parts of the...

Author(s): Oduor M. Aduol, Kenneth O. Ogila and Kamau John