Journal of
Medical Laboratory and Diagnosis

  • Abbreviation: J. Med. Lab. Diagn.
  • Language: English
  • ISSN: 2141-2618
  • DOI: 10.5897/JMLD
  • Start Year: 2010
  • Published Articles: 55

Full Length Research Paper

Safety of collagen and reticulin fibres in the liver and kidney of broiler chicken fed with Aspergillus niger -hydrolyzed cassava peel meal as carbohydrate source

Muhammed Abdurrasheed Ola
  • Muhammed Abdurrasheed Ola
  • Department of Histopathology, Faculty of Medical Laboratory Sciences, Usmanu Danfodiyo University, Sokoto, Nigeria.
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Adeyemo Isiah Adeyemi
  • Adeyemo Isiah Adeyemi
  • Biological Sciences Department, Ondo State University of Science and Technology, Okitipupa, Nigeria.
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Olutunde Oluwumi Abiola
  • Olutunde Oluwumi Abiola
  • Histopathology Unit, Pathology Department, University of Ilorin Teaching Hospital, Ilorin, Nigeria.
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Afodun Moyosore Adam
  • Afodun Moyosore Adam
  • Department of Anatomy, University of Ilorin, Ilorin, Nigeria.
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Ajayi Ayooye Samuel
  • Ajayi Ayooye Samuel
  • Department of Morbid Anatomy and Forensic Medicine, Usmanu Danfodiyo University Teaching Hospital, Sokoto, Nigeria.
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  •  Received: 14 November 2015
  •  Accepted: 14 March 2016
  •  Published: 31 March 2016


Cassava peels which is available in large quantity as waste is being considered as a possible replacement for maize in chicken feeds due to the world shortage of cereals. This study examined the safety of collagen and reticulin fibres in the liver and kidney of broiler chicken, when the Aspergillus niger-hydrolyzed cassava peel meal (HCPM) was used as diet carbohydrate. HCPM was used as replacement for maize in chicken feeds at 0, 25, 50, 75, and 100%, respectively and were fed for 42 days. The animals were sacrificed and the liver and kidney were excised and fixed immediately in 10% formol-saline for 48 h. The tissues were processed for paraffin embedding. Sections were cut at 3 microns, dried for 30 min at 60°C and were stained using both Gordon & Sweet and Masson’s Trichrome methods. The result showed the preservation of the reticulin and collagen fibres up to the 50% inclusion of HCPM as replacement for maize. The implication is that; the use of HCPM will make available for human consumption, half of the world maize cereals been consumed by the poultry birds.


Key words: Aspergillus niger, cassava peels, cellulolytic enzymes, collagen fibre, reticulin.