Journal of
Medicinal Plants Research

  • Abbreviation: J. Med. Plants Res.
  • Language: English
  • ISSN: 1996-0875
  • DOI: 10.5897/JMPR
  • Start Year: 2007
  • Published Articles: 3707

Full Length Research Paper

Chemical composition and biological potentials of Lebanese Cupressus sempervirens L. leaves’ extracts

Layal Anka
  • Layal Anka
  • Department of Life and Earth Sciences, Faculty of Sciences, Lebanese University, Lebanon.
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Hassan Rammal
  • Hassan Rammal
  • Doctoral School of Science and Technology, Research Platform for Environmental Sciences (PRASE), Lebanese University, Lebanon.
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Ahmad Kobeissi
  • Ahmad Kobeissi
  • Department of Life and Earth Sciences, Faculty of Sciences, Lebanese University, Lebanon.
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Hamid Bou Saab
  • Hamid Bou Saab
  • Department of Life and Earth Sciences, Faculty of Sciences, Lebanese University, Lebanon.
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  •  Received: 15 September 2019
  •  Accepted: 22 November 2019
  •  Published: 30 June 2020

Abstract

The essential oils and defatted ethanolic extracts isolated from Cupressus sempervirens L. leaves collected from two geographically distinct regions in Lebanon; Beit El-Dein (mountain) and Jbeil (sea-side) were analysed for their chemical composition by GC/MS and qualitative phytochemical screening, respectively. These were then tested for their in-vitro antioxidant and antibacterial activities. Fourteen and eighteen compounds were identified in Beit El-Dein and Jbeil samples, respectively with α-pinene (45.69-20.97%) and δ-3-carene (24.55-16.7%) representing the major constituents. C. sempervirens L. ethanolic extracts were able to reduce the stable radical 1,1-Diphenyl-2-picrylhydrazyl (DPPH) reaching 50% reduction with IC50 values equal to 113.17 and 155.75 μg/ml, respectively. These results positively correlated with the high phenol (77.4-66.4 mg gallic acid equivalent/g dry weight) and flavonoid (3.8-2.8 mg rutin equivalent/g dry weight) contents in both samples, respectively. Regarding the in-vitro antibacterial activity, essential oils exhibited a stronger potential mainly towards the Gram-positive bacteria Staphylococcus aureus compared with the ethanolic extracts as this was revealed by higher diameters of inhibition zone ranging between 7 and 12.3 mm and minimal inhibitory concentrations equal to 15.62 μl/ml. The results obtained from this study show that the Lebanese Cypress may be used as a good source of natural food preservatives and as drugs for various ailments.

Key words: Lebanese Cupressus sempervirens L., essential oil and ethanolic extract, antioxidant, antibacterial activities.