Journal of
Medicinal Plants Research

  • Abbreviation: J. Med. Plants Res.
  • Language: English
  • ISSN: 1996-0875
  • DOI: 10.5897/JMPR
  • Start Year: 2007
  • Published Articles: 3672

Full Length Research Paper

Identification of flavonol glycosides and in vitro photoprotective and antioxidant activities of Triplaris gardneriana Wedd

Macedo Sandra Kelle Souza
  • Macedo Sandra Kelle Souza
  • Federal University of San Francisco Valley Pernambuco, Brazil.
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Almeida Tamires dos Santos
  • Almeida Tamires dos Santos
  • Federal University of San Francisco Valley Pernambuco, Brazil.
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Ferraz Christiane Adrielly Alves
  • Ferraz Christiane Adrielly Alves
  • Federal University of San Francisco Valley Pernambuco, Brazil.
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Oliveira Ana Paula
  • Oliveira Ana Paula
  • Federal University of San Francisco Valley Pernambuco, Brazil.
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, Anjos Victor Hugo Almeida
  • , Anjos Victor Hugo Almeida
  • Federal Institute of Education, Science and Technology of Sertão Pernambucano, Pernambuco, Brazil.
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Siqueira Filho José Alves
  • Siqueira Filho José Alves
  • Centro Reference for Recovery of Degraded Areas of Caatinga (CRAD), Federal University of San Francisco Valley, Pernambuco, Brazil.
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Araújo Edigênia Cavalcante da Cruz
  • Araújo Edigênia Cavalcante da Cruz
  • Federal University of San Francisco Valley Pernambuco, Brazil.
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Almeida Jackson Roberto Guedes da Silva
  • Almeida Jackson Roberto Guedes da Silva
  • Federal University of San Francisco Valley Pernambuco, Brazil.
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Silva Naiane Darklei Santos
  • Silva Naiane Darklei Santos
  • Federal University of San Francisco Valley Pernambuco, Brazil.
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Nunes Xirley Pereira*
  • Nunes Xirley Pereira*
  • Federal University of San Francisco Valley Pernambuco, Brazil.
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  •  Received: 10 September 2014
  •  Accepted: 16 February 2015
  •  Published: 17 February 2015

Abstract

Triplaris gardneriana belongs to the family Polygonaceae, known for producing a number of biologically important molecules. The present study was aimed at identifying and quantify its total flavonoid content and determining the antioxidant and photoprotective potential of the plant’s leaves. The flavonoids present in the extract and fractions were analyzed using Liquid chromatography–mass spectrometry (LC-MS). The antioxidant activity was evaluated using 2,2-diphenyl-1-picrylhydrazyl (DPPH) free radical scavenging assay and autoxidation of β-carotene. The absorbance of the extracts was measured at different concentrations between 260 to 400 nm wavelengths. Calculation of sun protection factor (SPF) was determined using the formula developed by Mansur. The total flavonoid content was determined by the method developed by Dewanto. In the study, the following four flavonols were identified: quercetin-hexoside (2a), quercetin-pentoside (2b), quercetin-ramnobioside (2c) and myricetin-hexoside (2d). The crude ethanol extract, ethyl acetate, and methanol fractions showed higher flavonoid content and also exhibited excellent antioxidant and photoprotective activity. The SPF values were best observed for the crude ethanol extract and for the chloroform, ethyl acetate and methanol fractions. The good antioxidant and photoprotective potential can be attributed to the presence of flavonols identified for the first time in this species.

 

Key words: Triplaris gardneriana, Polygonaceae, antioxidant activity, photoprotective activity, glycosylated flavonols, LC-MS.