Journal of
Medicinal Plants Research

  • Abbreviation: J. Med. Plants Res.
  • Language: English
  • ISSN: 1996-0875
  • DOI: 10.5897/JMPR
  • Start Year: 2007
  • Published Articles: 3778

Full Length Research Paper

Ultrasound-assisted extraction of oxymatrine from Sophora flavescens

En-Qin Xia1, Hui-Ling Li1, Xiang-Rong Xu2, Fang Li1, Gui-Fang Deng1, Sha Li1, Shan Wu1 and Hua-Bin Li1*
1Guangdong Provincial Key Laboratory of Food, Nutrition and Health, School of Public Health, Sun Yat-Sen University, Guangzhou 510080, China. 2Key Laboratory of Marine Bio-resources Sustainable Utilization, South China Sea Institute of Oceanology, Chinese Academy of Sciences, Guangzhou 510301, China.
Email: [email protected]

  •  Accepted: 17 January 2012
  •  Published: 16 March 2012


Oxymatrine is one of main active compounds found in Sophora flavescens, which have been extensively used in China for the treatment of several diseases on viral hepatitis, cancer, cardiac and skin. The optimization of ultrasound-assisted extraction of oxymatrine from S. flavescens was investigated, and the extracts were analyzed by high-performance liquid chromatography (HPLC). Effects of several experimental parameters, such as concentration of extracting solvent, the ratio of liquid to material, extraction temperature, and extraction time on extraction efficiencies of oxymatrine from S. flavescens using ultrasound-assisted extraction were evaluated. The optimal extraction conditions were 1 g ground plant sample with 65% (v/v) ethanol solution in water, the ratio of liquid to material at 30:1 (ml/g) and extraction for 10 min at 50°C under ultrasonic irradiation. The extraction rate of oxymatrine using ultrasound-assisted extraction was higher than those obtained using other methods, such as conventional solvent extraction, decocting method, Soxhlet extraction and microwave-assisted extraction. The results indicated that ultrasound-assisted extraction is a very useful method, and helpful for the full utilization of S. flavescens.


Key words: Ultrasound, extraction, oxymatrine and Sophora flavescens.