Journal of
Medicinal Plants Research

  • Abbreviation: J. Med. Plants Res.
  • Language: English
  • ISSN: 1996-0875
  • DOI: 10.5897/JMPR
  • Start Year: 2007
  • Published Articles: 3707

Full Length Research Paper

Effect of auxin and cytokinin on phenolic content of Baccharis myriocephala DC. (Asteraceae) produced in vitro

Sharon Santos de Lima
  • Sharon Santos de Lima
  • Department of Pharmaceutical Sciences, Institute of Biological Sciences and Health, Federal Rural University of Rio de Janeiro, Brazil.
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Simone da Silva
  • Simone da Silva
  • Coordinating Plant Biotechnology, Amazon Biotechnology Center, Brazil.
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Claudio Barbosa Moreira
  • Claudio Barbosa Moreira
  • Department of Botany, Bioscience Institute, Federal University of the State of Rio de Janeiro, Brazil.
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Celso Luiz Salgueiro Lage
  • Celso Luiz Salgueiro Lage
  • National Institute of Industrial Property, Intellectual Property and Innovation Academy, Brazil.
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Alice Sato
  • Alice Sato
  • Department of Botany, Bioscience Institute, Federal University of the State of Rio de Janeiro, Brazil.
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  •  Received: 08 August 2017
  •  Accepted: 11 October 2017
  •  Published: 03 November 2017

Abstract

A protocol for large-scale in vitro production of Baccharis myriocephala was developed. Total phenolic content was analyzed in plants cultured on MS + 1.0 mg.L-1 of indoleacetic acid (IAA), benzyladenine (BA) or kinetin (KIN). Nodal segments obtained from seeds germinated in vitro on Murashige and Skoog basal medium (MS) were subcultured on MS basal medium, hormone-free or with different growth regulators added: IAA, BA or KIN. The total phenolic content was determined using the Folin-Denis spectrophotometric method, and the content of phenolics of extracts was expressed in terms of gallic acid-equivalent (µg GA/mg dry weight). The best growth and development of the plants was induced by MS + 1.0 mg.L-1 IAA, which stimulated the production of 23 new nodal segments from a single phytomer after 60 days of in vitro culture. However, MS + 1.0 mg.L-1 IAA accumulated the lowest phenolic content, and only 18.46 µg/mg dry weight. A significant negative correlation was noted, since the phenolic content increased when the dry weight decreased. This culture system is capable of supplying good-quality raw material in adequate quantity to meet the growing demand for medicinal plants. However, the accumulation of polyphenols is lower in cultures with higher dry weight.

 

Key words: Carqueja, micropropagation, growth regulators, phenolics accumulation.