Journal of
Medicinal Plants Research

  • Abbreviation: J. Med. Plants Res.
  • Language: English
  • ISSN: 1996-0875
  • DOI: 10.5897/JMPR
  • Start Year: 2007
  • Published Articles: 3704

Full Length Research Paper

In vivo TNF-α and IL-1β inhibitory activity of phenolics isolated from Trachelospermum jasminoides (Lindl.) Lem

Maha Salama*
  • Maha Salama*
  • Department of Pharmacognosy, Faculty of Pharmacy, Cairo University, Egypt.
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Seham El-Hawary
  • Seham El-Hawary
  • Department of Pharmacognosy, Faculty of Pharmacy, Cairo University, Egypt.
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Ola Mousa
  • Ola Mousa
  • Department of Pharmacognosy, Faculty of Pharmacy, Al-Ahram Canadian University, Egypt.
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Noha El- Askary
  • Noha El- Askary
  • Department of Pharmacognosy, Faculty of Pharmacy, Al-Ahram Canadian University, Egypt.
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Ahmed Esmat
  • Ahmed Esmat
  • Department of Pharmacology and Toxicology, Faculty of Pharmacy, Ain Shams University, Egypt.
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  •  Received: 25 November 2014
  •  Accepted: 29 December 2014
  •  Published: 10 January 2015

Abstract

A bio-guided fractionation of the defatted ethanolic extract (DEE) of the aerial parts of Trachelospermum jasminoides and its fractions: ethyl acetate fraction (EAF), chloroform fraction (CF) and n-butanol fraction (BF) were carried out, using carragenan induced rat paw edema method, to evaluate the in vivo acute anti-inflammatory potential at a given dose of 100 mg/kg body weight compared to indomethacin (20 mg/kg body weight). The EAF revealed the highest anti-inflammatory activity (76.92%) relative to the DEE and the CF (63.82 and 48.75%, respectively) at the same tested dose, while the BF was significantly inactive. The EAF and its major isolated compounds were investigated to determine the level of pro-inflammatory cytokines using enzyme-linked immunosorbant assay (ELISA). Seven major compounds were isolated from the EAF, identified as: trachelogenin, nor-trachelogenin and tracheloside, in addition to apigenin, luteolin, quercetin and luteolin-7-O-β-D-glucopyranoside. Identification of the isolated compounds was achieved by their physico-chemical properties and spectral analysis (1D and 2D NMR). The EAF and the isolated compounds inhibited the excessive production of tumor necrosis factor-alpha (TNF-α) and interleukin (IL)-1β. Furthermore, the liquid chromatography/electrospray ionization-tandem mass spectrometry (LC/ESI-MS) for the bioactive EAF was carried out to complete the phytochemical picture. The results of this study verified that the EAF and the major compounds exert their action through inhibition of TNF-α and IL-1 β.

 

Key words: Trachelospermum jasminoides, tumor necrosis factor-alpha (TNF-α), interleukin (IL)-1β, liquid chromatography/electrospray ionization-tandem mass spectrometry (LC/ESI-MS).