Journal of
Medicinal Plants Research

  • Abbreviation: J. Med. Plants Res.
  • Language: English
  • ISSN: 1996-0875
  • DOI: 10.5897/JMPR
  • Start Year: 2007
  • Published Articles: 3835

Full Length Research Paper

Analysis of total flavonoids and phenolics in different fractions of bark and needle extracts of Pinus roxburghii and Pinus wallichiana

Alya Maimoona1*, Ismat Naeem2, Zeb Saddiqe1, Niaz Ali3, Ghayour Ahmed4 and Ismail Shah4
1Department of Botany, Lahore College for Women University Lahore, Pakistan. 2Department of Chemistry, Lahore College for Women University Lahore Pakistan. 3Institute of Basic Medical Sciences (IBMS) Khyber Medical University (KMU), Peshawar. 4Department of Pharmacy, University of Malakand, Chakdara, Khyberpakhtoonkhwa, Pakistan.
Email: [email protected]

  •  Accepted: 22 March 2011
  •  Published: 04 July 2011


Total flavonoid and phenolic content was estimated quantitatively by using colorimetric method in various fractions of bark and needle extracts of Pinus roxburghii and Pinus wallichiana. Flavonoids may exist as free aglycone, but usually they are bound to sugar as glycosides and any one aglycone may occur in single plant in several combinations. For the measurement of total flavonoid content, original plant extract was hydrolysed and aglycone was measured before and after hydrolysis to calculate the presence of free aglycone content.  Flavonoid contents (aglycone) of the extracts, in terms of quercetin equivalent (the standard curve equation: y = 0.0877x -0.0595, r2 = 0.9981), were in the range of 60.1 ± 4.5 to 484 ± 2.5 before and after hydrolysis. As such, the values fell in the range of 76.9 ± 3.2 to 943 ± 2.6. The total phenol varied from 394 + 0.03 to 1331 + 0.24 measured in terms of gallic acid equivalent (with standard equation: y = 0.0197x-0.0076, r2 = 0.9997).


Key words: Flavonoid, phenolics, aglycone, Pinus wallichianaPinus roxburghii.