Journal of
Medicinal Plants Research

  • Abbreviation: J. Med. Plants Res.
  • Language: English
  • ISSN: 1996-0875
  • DOI: 10.5897/JMPR
  • Start Year: 2007
  • Published Articles: 3631

Full Length Research Paper

Evaluation of cytotoxic activity of triterpenes from Clusia studartiana

Lavínia de C. Brito
  • Lavínia de C. Brito
  • Laboratório de Química de Produtos Naturais, Instituto de Tecnologia em Fármacos, Fiocruz, Rio de Janeiro, RJ, Brasil.
  • Google Scholar
Marcia V. de Carvalho
  • Marcia V. de Carvalho
  • Laboratório de Farmacologia Molecular, Instituto de Tecnologia em Fármacos, Fiocruz, Rio de Janeiro, RJ, Brasil.
  • Google Scholar
Vagner P. da Silva
  • Vagner P. da Silva
  • Laboratório de Química de Produtos Naturais, Instituto de Tecnologia em Fármacos, Fiocruz, Rio de Janeiro, RJ, Brasil.
  • Google Scholar
Alan P. Heringer
  • Alan P. Heringer
  • Laboratório de Química de Produtos Naturais, Instituto de Tecnologia em Fármacos, Fiocruz, Rio de Janeiro, RJ, Brasil.
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Priscila M. da Silva
  • Priscila M. da Silva
  • Laboratório de Farmacologia Molecular, Instituto de Tecnologia em Fármacos, Fiocruz, Rio de Janeiro, RJ, Brasil.
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Ana Paula G. A. Fontao
  • Ana Paula G. A. Fontao
  • Laboratório de Farmacologia Molecular, Instituto de Tecnologia em Fármacos, Fiocruz, Rio de Janeiro, RJ, Brasil.
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Maria R. Figueiredo
  • Maria R. Figueiredo
  • Laboratório de Química de Produtos Naturais, Instituto de Tecnologia em Fármacos, Fiocruz, Rio de Janeiro, RJ, Brasil.
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André Luiz F. Sampaio
  • André Luiz F. Sampaio
  • Laboratório de Farmacologia Molecular, Instituto de Tecnologia em Fármacos, Fiocruz, Rio de Janeiro, RJ, Brasil.
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  •  Received: 02 June 2019
  •  Accepted: 30 July 2019
  •  Published: 31 August 2019

Abstract

Family Clusiaceae is spread throughout tropical as well as temperate zones, presenting many triterpenes, reported as selective antitumoral agents. The aim of this study is to isolate and identify triterpenes from Clusia studartiana C. M. Vieira & Gomes da Silva and evaluate their cytotoxicity in melanoma (SKMEL 28) and myeloid leukemia (K562) cell lines. Thus, the hexanic extract was processed by phytochemical methods to isolate and purify the pentacyclic triterpenes: 3-oxo-friedelin (1), 3-β-hydroxy-friedelin (2) and 3-oxo-olean-12-en-28-oic-acid (3), identified by spectroscopic methods. Cytotoxicity was evaluated by MTT assay, apoptosis/necrosis by Annexin V/PI, caspase activity by FLICA in flow cytometry and P-gp modulation was measured by interference in the efflux of Rhodamine 123. From the results,  triterpenes 2 and 3 showed inhibitory effect in K562 cells proliferation, and only the compound  3 was able to increase percentage of AnnexinV+PI- in cells (p<0.05), with 40% increase in caspase 3/7+ cells and showed inhibition on P-gp activity.

Key words: Apoptosis, caspase, Clusiaceae, Clusia studartiana, cytotoxicity, multidrug resistance.