Family Clusiaceae is spread throughout tropical as well as temperate zones, presenting many triterpenes, reported as selective antitumoral agents. The aim of this study is to isolate and identify triterpenes from Clusia studartiana C. M. Vieira & Gomes da Silva and evaluate their cytotoxicity in melanoma (SKMEL 28) and myeloid leukemia (K562) cell lines. Thus, the hexanic extract was processed by phytochemical methods to isolate and purify the pentacyclic triterpenes: 3-oxo-friedelin (1), 3-β-hydroxy-friedelin (2) and 3-oxo-olean-12-en-28-oic-acid (3), identified by spectroscopic methods. Cytotoxicity was evaluated by MTT assay, apoptosis/necrosis by Annexin V/PI, caspase activity by FLICA in flow cytometry and P-gp modulation was measured by interference in the efflux of Rhodamine 123. From the results, triterpenes 2 and 3 showed inhibitory effect in K562 cells proliferation, and only the compound 3 was able to increase percentage of AnnexinV+PI- in cells (p<0.05), with 40% increase in caspase 3/7+ cells and showed inhibition on P-gp activity.
Key words: Apoptosis, caspase, Clusiaceae, Clusia studartiana, cytotoxicity, multidrug resistance.
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