Journal of
Medicinal Plants Research

  • Abbreviation: J. Med. Plants Res.
  • Language: English
  • ISSN: 1996-0875
  • DOI: 10.5897/JMPR
  • Start Year: 2007
  • Published Articles: 3835

Full Length Research Paper

Determination of cyclic oligomers residues in tea catechins isolated by polyamide-6 column

Jianhui Ye1, Nana Li1, Huiling Liang1,3, Junjie Dong1, Jianliang Lu2, Xinqiang Zheng2and Yuerong Liang1*
1Tea Research Institute, Zhejiang University, 268 Kaixuan Road, Hangzhou 310029, China. 2The State Agriculture Ministry Laboratory of Horticulture Plant Growth Development and Quality Improvement, Hangzhou 310058, China. 3South China Agricultural University, College of Horticulture, Guangzhou 510642, China.
Email: [email protected]

  •  Accepted: 05 April 2011
  •  Published: 04 July 2011


Cyclic oligomers ranging from tetramer to octamer and a tetramer plus –NH(CH2)2CHin ethanol extract of polyamide-6 (PA) were identified by HPLC/MS and MS/MS. An HPLC method was developed to detect the residues of the cyclic oligomers in tea catechins isolated by PA column. It showed that catechins had interference on HPLC analysis of the cyclic oligomers, but the interference could be eliminated using polyvinylpolypyrrolidone as an adsorbent to remove catechins in the sample. It is found that pretreatment of PA using 95% (v/v) ethanol was an effective method to eliminate cyclic oligomers residues in the final products isolated by PA column.


Key words: Tea catechins, polyamide-6, cyclic oligomer, residue, high-performance liquid chromatography (HPLC), mass spectrometry (MS).