Journal of
Medicinal Plants Research

  • Abbreviation: J. Med. Plants Res.
  • Language: English
  • ISSN: 1996-0875
  • DOI: 10.5897/JMPR
  • Start Year: 2007
  • Published Articles: 3758

Full Length Research Paper

Thin layer chromatography (TLC) technique in the investigation of artemisinin production in Artemisia annua L. medicinal plant hairy roots

Mohammad Bagher Pasha Zanousi1*, Tayebe Soleimani1, Mehrnaz Keyhanfar2, Saeed Shirali3 and Mahdi Raeesi4
1Department of Chemistry, Chalous Branch, Islamic Azad University, Chalous, Iran. 2Department of Biotechnology, Faculty of Advanced Sciences and Technologies, The University of Isfahan, Isfahan, 81746-73441, Iran. 3Department of Laboratory Sciences, Chalous Branch, Islamic Azad University, Chalous, Iran. 4Young Researchers Club, Islamic Azad University, Chalous Branch, Chalous, Iran.
Email: [email protected]

  •  Accepted: 26 January 2012
  •  Published: 16 March 2012

Abstract

Artemisia annua L. is an important medicinal plant, which is used for the treatment of malaria. Artemisinin is the active secondary metabolite in this plant and it is a sesquiterpen. This plant was studied for artemisinin production in hairy root induced by Agrobacterium rhizogenes. Polymerase chain reaction (PCR) for rolB gene confirmed the transformed hairy roots induced by A. rhizogenes. Then, thin layer chromatography (TLC) was employed for investigating the characteristics and quality of artemisinin in extracts of hairy roots and control roots of A. annua. Result of TLC showed the presence of artemisinin in the induced hairy roots.

 

Key words: Artemisia annuaAgrobacterium rhizogenes, hairy root, thin layer chromatography (TLC) technique and artemisinin.