Journal of
Medicinal Plants Research

  • Abbreviation: J. Med. Plants Res.
  • Language: English
  • ISSN: 1996-0875
  • DOI: 10.5897/JMPR
  • Start Year: 2007
  • Published Articles: 3843

Full Length Research Paper

Antifungal and cytotoxicity activities of Anacardium othonianum extract

Flávia Maria Lígia Marangoni Jordão Curado
  • Flávia Maria Lígia Marangoni Jordão Curado
  • Center for Research in Exact and Technological Sciences, University of Franca. Av. Dr. Armando Salles de Oliveira, 201, CEP 14404-600, Franca, São Paulo, Brazil.
  • Search for this author on:
  • Google Scholar
Ana Paula Gazolla
  • Ana Paula Gazolla
  • Seed Laboratory, Federal Institute of Education, Science and Technology of Goiano– Rio Verde. Rodovia Sul Goiana, km 1-Zona Rural, CEP 75901-970, Rio Verde, of Goiano, Brazil.
  • Search for this author on:
  • Google Scholar
Rita Cassia Nascimento Pedroso
  • Rita Cassia Nascimento Pedroso
  • Center for Research in Exact and Technological Sciences, University of Franca. Av. Dr. Armando Salles de Oliveira, 201, CEP 14404-600, Franca, São Paulo, Brazil.
  • Search for this author on:
  • Google Scholar
Letícia Pereira Pimenta
  • Letícia Pereira Pimenta
  • Center for Research in Exact and Technological Sciences, University of Franca. Av. Dr. Armando Salles de Oliveira, 201, CEP 14404-600, Franca, São Paulo, Brazil.
  • Search for this author on:
  • Google Scholar
Pollyanna Francielli de Oliveira
  • Pollyanna Francielli de Oliveira
  • Center for Research in Exact and Technological Sciences, University of Franca. Av. Dr. Armando Salles de Oliveira, 201, CEP 14404-600, Franca, São Paulo, Brazil.
  • Search for this author on:
  • Google Scholar
Denise Crispim Tavares
  • Denise Crispim Tavares
  • Center for Research in Exact and Technological Sciences, University of Franca. Av. Dr. Armando Salles de Oliveira, 201, CEP 14404-600, Franca, São Paulo, Brazil.
  • Search for this author on:
  • Google Scholar
Márcio Luis Andrade e Silva
  • Márcio Luis Andrade e Silva
  • Center for Research in Exact and Technological Sciences, University of Franca. Av. Dr. Armando Salles de Oliveira, 201, CEP 14404-600, Franca, São Paulo, Brazil.
  • Search for this author on:
  • Google Scholar
Wilson Roberto Cunha
  • Wilson Roberto Cunha
  • Center for Research in Exact and Technological Sciences, University of Franca. Av. Dr. Armando Salles de Oliveira, 201, CEP 14404-600, Franca, São Paulo, Brazil.
  • Search for this author on:
  • Google Scholar
Rosemeire Cristina Linhari Rodrigues Pietro
  • Rosemeire Cristina Linhari Rodrigues Pietro
  • Department of Drugs and Medicines, School of Pharmaceutical Sciences, UNESP-Univ. Estadual Paulista, Rodovia Araraquara-Jau, km 1, CEP 14801-902, Araraquara, São Paulo, Brazil.
  • Search for this author on:
  • Google Scholar
Ana Helena Januário
  • Ana Helena Januário
  • Center for Research in Exact and Technological Sciences, University of Franca. Av. Dr. Armando Salles de Oliveira, 201, CEP 14404-600, Franca, São Paulo, Brazil.
  • Search for this author on:
  • Google Scholar
Patrícia Mendonça Pauletti
  • Patrícia Mendonça Pauletti
  • Center for Research in Exact and Technological Sciences, University of Franca. Av. Dr. Armando Salles de Oliveira, 201, CEP 14404-600, Franca, São Paulo, Brazil.
  • Search for this author on:
  • Google Scholar
Juliana de Fátima Sales
  • Juliana de Fátima Sales
  • Seed Laboratory, Federal Institute of Education, Science and Technology of Goiano– Rio Verde. Rodovia Sul Goiana, km 1-Zona Rural, CEP 75901-970, Rio Verde, of Goiano, Brazil.
  • Search for this author on:
  • Google Scholar
Fabiano Guimarães Silva
  • Fabiano Guimarães Silva
  • Plant Tissue Culture Laboratory, Federal Institute of Education, Science and Technology of Goiano– Rio Verde. Rodovia Sul Goiana, km 1-Zona Rural, CEP 75901-970, Rio Verde, Goiano,Brazil.
  • Search for this author on:
  • Google Scholar


  •  Received: 31 March 2016
  •  Accepted: 18 July 2016
  •  Published: 03 August 2016

Abstract

The antifungal activities of the crude ethanolic extract of Anacardium othonianum (Anacardiaceae) leaves (EE) and the n-hexane (HF), EtOAc (EF), n-BuOH (BF) and hydromethanolic (HMF) fractions were assayed against Candida albicans (ATCC 64548) and Trichophyton rubrum (Tr1). Additionally, the cytotoxicities were also evaluated against normal human lung fibroblasts (GM07492A). The EE yielded minimum inhibitory concentration (MIC) values of 78.12 and 312.50 µg/mL for C. albicans and T. rubrum, respectively, and no cytotoxicity was observed. The EF and BF fractions exhibited enhanced antifungal activities when compared with the MIC values obtained for the EE fraction, and no cytotoxicity was observed for either fraction. Thus, the EF fraction, which displayed the higher antifungal activity, was purified, leading to the isolation of the following compounds: amentoflavone (1), gallic acid (2), protocatechuic acid (3), and ethyl 3,4,5-trimethoxybenzoate (4). HPLC analysis confirmed the presence of compounds 1-4 and 1-3 in the EF and BF fractions, respectively, in different proportions. The results suggest that the antifungal activities of the EE, EF and BF fractions may be attributed mainly to the actions of 1, 2 and 3.

Key words: Anacardium othonianum extract, antifungal and cytotoxicity activities, HPLC analysis.

Copyright © 2025 Author(s) retain the copyright of this article.

This article is published under the terms of the Creative Commons Attribution License 4.0

Back to Vol. 10 No. 29
Back to articles
SUBSCRIBE TO RSS
SUBMIT MANUSCRIPT
CONFERENCES