Journal of
Medicinal Plants Research

  • Abbreviation: J. Med. Plants Res.
  • Language: English
  • ISSN: 1996-0875
  • DOI: 10.5897/JMPR
  • Start Year: 2007
  • Published Articles: 3835

Full Length Research Paper

Doubling the chromosome number of Salvia hains using colchicine: Evaluation of morphological traits of recovered plants

Mohammad Sadat Hosseini Grouh1*, Heidar Meftahizade2, Naser Lotfi3, Vahid Rahimi4 and Behdad Baniasadi1
1Department of Plant Sciences, Faculty of Agriculture, University of Jiroft, Jiroft, Iran. 2ACECR Medicinal Plants Research Center, Ilam, Iran. 3Department of Medicinal Plants, College of Miandoab, University of Uremia, Uremia, Iran. 4Department of Horticulture, Abouraihan Campus, University of Tehran, Tehran, Iran.
Email: [email protected], [email protected]

  •  Accepted: 20 July 2011
  •  Published: 23 September 2011


This study demonstrates a protocol of polyploidy induction in Salvia hains, in order obtained tetraploid five concentrations (0.1, 0.3, 0.4, 0.5 and 0.7%) of colchicine were used to determine the best treatment for the induction of tetraploid plants. Treatment seeds with colchicine for 24 h product tetraploid plants. Ploidy level of the resulting plantlets was determined by morphological study, chromosome counting and flow cytometry. Tetraploid plants were obtained from S. hains after applied concentrations 0.3, 0.4 and 0.5% of colchicine. Plants obtained from 0.1% colchicine were all diploids. The induced tetraploid in S. hains was accompanied by larger stomata increase in chloroplast number in guard cells and decrease in stomata density, compared to diploid (control) plants. The techniques used to induce tetraploid S. hains plants by colchicine were successful and could be used in breeding programs.


Key words: Autotetrapolid, flowcytometry, polyploid, Salvia hains, chromosome counting.