Journal of
Medicinal Plants Research

  • Abbreviation: J. Med. Plants Res.
  • Language: English
  • ISSN: 1996-0875
  • DOI: 10.5897/JMPR
  • Start Year: 2007
  • Published Articles: 3834

Full Length Research Paper

Nutritional, phytochemical potential and pharmacological evaluation of Nigella Sativa (Kalonji) and Trachyspermum Ammi (Ajwain)

Shabnam Javed1*, Ahmad Ali Shahid1, Muhammad Saleem Haider1, Aysha Umeera1, Rauf Ahmad2 and Sobia Mushtaq1
1Institute of Agricultural Sciences, University of the Punjab, Quaid-e- Azam Campus, Lahore-54590 Lahore. Pakistan. 2Pakistan Council of Scientific and Industrial Research, Lahore, Pakistan.
Email: [email protected]

  •  Accepted: 14 December 2011
  •  Published: 09 February 2012


Nigella sativa and Trachyspermum ammi were screened for phytochemical constituents and nutritional analysis. Tests for flavonoids, tannins, steroids were positive in both spices except saponins. The nutrition analysis indicated higher energy value (459.29 and 314.55%), carbohydrates (39.04 and 47.54%), protein content (24.05 and 20.23%), fat content (21.67 and 4.83%), moisture content (5.4 and 11.6%), fibre content (5.5 and 4.3%) and ash content (4.34 and 11.5%) in N. sativa and T. ammi seeds, respectively. Aqueous, methanolic, ethanolic, n-Hexane extracts of N. sativa and T. ammi and also essential oil of T. ammi were subjected toin vitro antifungal and antibacterial assay. Micro well dilution assay was adopted against three human pathogenic fungal strains Aspergillus flavus, Aspergillus niger, Candida albicans andfour bacterial isolates Escherichia coli, Staphylococcus sp., Pseudomonas syringae and Bacillus subtilis. Among all the four extracts of N. sativa testedmethanolic extract showed maximum inhibitory potential against all the test fungi and bacteriaIn case of Tammi extracts and essential oil, all the aqueous, organic extracts especially ethanol, n-hexane and essential oil depicted marked antimicrobial potential against all pathogens. Anti bacterial activity of n-Hexane extract was maximum for all bacteria (4.76 cm) P. syringica, (4.03 cm) B. subtilis, (3.73 cm) E. coli and (4.76 cm) S. sp. Bactericidal action of different fractions of T. ammidecrease in the order n-Hexane extract>essential oil>ethanol extract>methanol extract>aqueous extract.


Key words: Nigella sativaTrachyspermum ammi, phytochemical analysis, nutrition value, antifungal/antibacterial.