Journal of
Medicinal Plants Research

  • Abbreviation: J. Med. Plants Res.
  • Language: English
  • ISSN: 1996-0875
  • DOI: 10.5897/JMPR
  • Start Year: 2007
  • Published Articles: 3804

Full Length Research Paper

Study on the extraction process and tyrosinase inhibition property of cichoric acid in Echinacea purpurea L.

Ling Jiang, Fang Hu, Yuling Tai, Xiumei Yang, Deqiang Yu, Dahui Li and Yi Yuan*
College of Life Sciences, Anhui Agricultural University, Botany Laboratory, Hefei Anhui, China.
Email: [email protected]

  •  Accepted: 22 November 2011
  •  Published: 17 October 2012


Echinacea purpurea has become a commonly used herbal medicine because it contains various biologically active compounds, such as cichoric acid. Extraction conditions of cichoric acid from the flowers of E. purpurea L. were investigated through an orthogonal design of L(34) assay. For purification, the extract was adsorbed on an AB-8 macroporous resin and eluted with 50% ethanol, then extracted with ethyl acetate four times, resulting in a residue containing 60.23% cichoric acid. Tyrosinase inhibition property of cichoric acid extracts was tested compared with arbutin by Spectrophotometer method. The extraction was optimized by adding 40% ethanol at a ratio of 1:15 (g/mL) to extract three times at 70ï‚°C for 1 h. The cichoric acid extracts from E. purpurea was found to have significant tyrosinase inhibition activity in broad range of concentration (≥10 mg/mL).


Key words: Echinacea purpurea L., cichoric acid; extraction, orthogonal design, antimicrobial activity.