Journal of
Medicinal Plants Research

  • Abbreviation: J. Med. Plants Res.
  • Language: English
  • ISSN: 1996-0875
  • DOI: 10.5897/JMPR
  • Start Year: 2007
  • Published Articles: 3806

Full Length Research Paper

Anti-aging effect of black rice against H2O2-induced premature senescence

Mi Jin Choi1, Hyun Young Kim2 and Eun Ju Cho1*
1Department of Food Science and Nutrition, Pusan National University, 30 Jangjeon-dong, Geumjeong-gu, Busan 609-735, Republic of Korea. 2Deptartment of Food Science, Gyeongnam National University of Science and Technology, 150 chilamdong, Jinju 660-758, Republic of Korea.
Email: [email protected]

  •  Accepted: 13 March 2012
  •  Published: 30 May 2012


This study was focused on the anti-aging effect of black rice under stress-induced premature senescence (SIPS) of WI-38 human diploid fibroblasts cells caused by hydrogen peroxide (H2O2), a well-established experimental model of cellular aging. The protective effect of the MeOH extract from black rice against H2O2-induced premature senescence was investigated using WI-38 cells by evaluating the cell viability, lipid peroxidation and cell life span. H2O2-treated WI-38 cells exhibited the phenomena of SIPS, the loss of cell viability, the increase of lipid peroxidation and shortening of the cell lifespan. However, the treatment with black rice attenuated cellular oxidative stress through increase in the cell viability and inhibition in lipid peroxidation. In addition, the life span of WI-38 cell showed extension of the population doubling level, suggesting that it would delay aging process. These results suggest that the MeOH extract from black rice may delay the aging process by attenuation of oxidative stress under SIPS cellular model.


Key words: Black rice, ageing, WI-38 cell, premature senescence, oxidative stress.