Antileishmanial effects of the alkaloid-rich fraction of Quassia amara L.

Renata S. Gabriel; Ana Claudia F. Amaral; Suzana Corte-Real; Rosana C. Lopes; Celuta S. Alviano; Alane B. Vermelho; Daniela S. Alviano; Igor A. Rodrigues

doi:10.5897/JMPR2016.6253

Journal of
Medicinal Plants Research

Abbreviation: J. Med. Plants Res.
Language: English
ISSN: 1996-0875
DOI: 10.5897/JMPR
Start Year: 2007
Published Articles: 3843

Full Length Research Paper

Antileishmanial effects of the alkaloid-rich fraction of Quassia amara L.

Renata S. Gabriel

Renata S. Gabriel
Graduate Program in Science (Microbiology), Institute of Microbiology Paulo de GÃ³es, Federal University of Rio de Janeiro, 21941-902, RJ, Brazil.
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Ana Claudia F. Amaral

Ana Claudia F. Amaral
Department of Natural Products, Farmaguinhos, FIOCRUZ, 21041-250, RJ, Brazil.
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Suzana Corte-Real

Suzana Corte-Real
Laboratory of Structural Biology and Electron Microscopy Platform Rudolf Barth, Oswaldo Cruz Institute (IOC), 21040-360, RJ, Brazil.
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Rosana C. Lopes

Rosana C. Lopes
Department of Botany, Institute of Biology, Federal University of Rio de Janeiro, 21941-902, RJ, Brazil.
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Celuta S. Alviano

Celuta S. Alviano
Department of General Microbiology, Institute of Microbiology Paulo de GÃ³es, Federal University of Rio de Janeiro, 21941-902, RJ, Brazil.
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Alane B. Vermelho

Alane B. Vermelho
Department of General Microbiology, Institute of Microbiology Paulo de GÃ³es, Federal University of Rio de Janeiro, 21941-902, RJ, Brazil.
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Daniela S. Alviano

Daniela S. Alviano
Department of General Microbiology, Institute of Microbiology Paulo de GÃ³es, Federal University of Rio de Janeiro, 21941-902, RJ, Brazil.
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Igor A. Rodrigues

Igor A. Rodrigues
Department of Natural Products and Food, Faculty of Pharmacy, Federal University of Rio de Janeiro, 21941-902, RJ, Brazil.
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Article Number - 4E7BF1C61598
Vol.10(42), pp. 775-782 , November 2016
https://doi.org/10.5897/JMPR2016.6253

Received: 07 September 2016
Accepted: 03 November 2016
Published: 10 November 2016

Abstract

Control of leishmaniasis remains a challenge due the high toxicity of the chemotherapeutic drugs presently available. The ongoing search for better leishmanicidal compounds has brought herbal drugs into the limelight as safe and effective substitutes to conventional therapies which have various drawbacks. The current study was designed to evaluate the antileishmanial effect of Quassia amara against Leishmania amazonensis and Leishmania infantum. Different fractions (Hexane (Q1), dichloromethane (Q2), ethyl acetate (Q3) and butanol (Q4)) obtained from the liquid-liquid partition of the crude Q. amara methanol extract were tested against Leishmania promastigote forms. After 120 h of treatment with Q. amara fractions (1 to 500 µg/ml), the minimal inhibitory concentration (MIC) was determined using resazurin. The most active fraction (Q2) was analyzed using thin layer chromatography (TLC) and high performance liquid chromatography-diode array detector (HPLC-DAD) techniques. The effects of Q2 on parasite ultrastructure were investigated by transmission electron microscopy. In addition, Leishmania-infected macrophages were treated with Q2 in order to evaluate the anti-amastigote effect. Among the fractions tested, Q2 showed the highest activity against L. amazonensis and L. infantum promastigotes MIC values of 62.5 and 31.25 µg/ml, respectively. There were ultrastructural alterations, such as nuclear chromatin condensation, intense vacuolization and autophagic and myelin-like figures in parasites treated with Q2 (62.5 µg/ml). Macrophages previously infected with L. amazonensis and L. infantum promastigotes showed a drastic reduction in the number of parasites recovered in the supernatant after Q2 treatment at MIC values. The TLC and HPLC fingerprints of Q2 showed that alkaloids were the main chemical constituents in this fraction. The results presented herein showed that the alkaloid-rich fraction Q2 is a promising source of antileishmanial agents. Further investigation will be necessary in order to isolate and test the substance(s) responsible for the bioactivity.