Journal of
Medicinal Plants Research

  • Abbreviation: J. Med. Plants Res.
  • Language: English
  • ISSN: 1996-0875
  • DOI: 10.5897/JMPR
  • Start Year: 2007
  • Published Articles: 3849

Full Length Research Paper

Chemical constituents, antioxidant and antidiabetic activities of Cameroonian brown propolis sample

Paul Sakava
  • Paul Sakava
  • Department of Chemistry, Higher Teacher Training College, The University of Bamenda, P. O. Box 39 Bambili, Cameroon.
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Isaac S. Gade
  • Isaac S. Gade
  • Department of Chemistry, Faculty of Science, University of Maroua, P. O. Box 814: Maroua, Cameroon.
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Albert Atangana Fouda
  • Albert Atangana Fouda
  • Department of Chemistry, Faculty of Science, University of Maroua, P. O. Box 814: Maroua, Cameroon.
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Lanvin E. F. Edoun
  • Lanvin E. F. Edoun
  • Department of Biochemistry, Faculty of Science, University of Yaounde I, P. O. Box 812: Yaoundé, Cameroon.
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Dupon A. B. Ambamba
  • Dupon A. B. Ambamba
  • Department of Biochemistry, Faculty of Science, University of Yaounde I, P. O. Box 812: Yaoundé, Cameroon.
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Angelbert F. Awantu
  • Angelbert F. Awantu
  • Department of Chemistry, Faculty of Science, University of Bamenda, P. O. Box 39: Bambili, Cameroon.
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Emmanuel Talla
  • Emmanuel Talla
  • Department of Chemistry, Faculty of Science, University of Ngaoundere, P. O. Box 454: Ngaoundere, Cameroon
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Maurice F. Tagatsing
  • Maurice F. Tagatsing
  • Natural Substances and Valorization Laboratory, Department of Organic Chemistry, University of Yaoundé I, Faculty of Science, P. O. Box 812 Yaoundé, Cameroon.
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Théodore Alex. D. Atchadé
  • Théodore Alex. D. Atchadé
  • Natural Substances and Valorization Laboratory, Department of Organic Chemistry, University of Yaoundé I, Faculty of Science, P. O. Box 812 Yaoundé, Cameroon.
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Sophie Laurent
  • Sophie Laurent
  • Department of General, Organic and Biomedical Chemistry, Faculty of Medicine and Pharmacy, University of Mons, NMR and Molecular Imaging Laboratory, B-7000 Mons, Belgium.
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Celine Henoumont
  • Celine Henoumont
  • Department of General, Organic and Biomedical Chemistry, Faculty of Medicine and Pharmacy, University of Mons, NMR and Molecular Imaging Laboratory, B-7000 Mons, Belgium.
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  •  Received: 19 February 2025
  •  Accepted: 28 April 2025
  •  Published: 31 May 2025

Abstract

The n-hexane, ethyl acetate and methanol extracts of propolis were studied and yielded seven compounds which were characterized as 1-methyloctyl-1,8-didodecanoate (1), lupeol (2), lupenone (3), 12-dien-3-one (4), 3-oxo-11α-hydroxy-olean-12-ene (5), 12-oleanene-3,11-dione (6) and 3-oxofriedelan-28-al (7) using 1D and 2D NMR spectroscopic techniques. Compound 1 is new, while compounds 4, 5, 6 and 7 were isolated from propolis for the first time. Antioxidant activity was assessed by DPPH and FRAP. The IC50 of n-hexane extract was higher than that of methanol extract for DPPH (12.44 mg/mL versus 2.4 mg/mL).  Both n-hexane and methanol extracts reduced Fe3+ (20.82% versus 27.21%).  The ability of lupenone to bind glucose was assessed, followed by its ability to mimic the action of insulin through glucose uptake. Lupenone showed an ability to bind glucose. Its addition at 100 µg/mL increased glucose absorption. Propolis n-hexane and methanol extracts have antioxidant properties. The lupenone contained in propolis is a hypoglycemic agent and could contribute to the management of T2D.

Key words: Propolis, extracts, characterization, glucose uptake, antioxidant activity and diabetes.

Copyright © 2025 Author(s) retain the copyright of this article.

This article is published under the terms of the Creative Commons Attribution License 4.0

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