Journal of
Medicinal Plants Research

  • Abbreviation: J. Med. Plants Res.
  • Language: English
  • ISSN: 1996-0875
  • DOI: 10.5897/JMPR
  • Start Year: 2007
  • Published Articles: 3835

Full Length Research Paper

In vitro antibacterial activities of pomegranate extract against standard microorganisms of bovine mastitis

Giovanna Melatti Bermal Moreira
  • Giovanna Melatti Bermal Moreira
  • Universidade Estadual do Norte do Paraná – Brazil
  • Google Scholar
Leopoldo Sussumu Matsumoto
  • Leopoldo Sussumu Matsumoto
  • Universidade Estadual do Norte do Paraná – Brazil
  • Google Scholar
Marcelo Alves da Silva
  • Marcelo Alves da Silva
  • Universidade Estadual do Norte do Paraná – Brazil
  • Google Scholar
Luciana Pereira Silva
  • Luciana Pereira Silva
  • Universidade Estadual do Norte do Paraná – Brazil
  • Google Scholar
Regildo Márcio deGonçalvez Silva
  • Regildo Márcio deGonçalvez Silva
  • Universidade Estadual do Norte do Paraná – Brazil
  • Google Scholar
Erika Cosendey Toledo de Mello Peixoto*
  • Erika Cosendey Toledo de Mello Peixoto*
  • Universidade Estadual do Norte do Paraná – Brazil
  • Google Scholar


  •  Received: 21 May 2015
  •  Accepted: 14 September 2015
  •  Published: 25 September 2015

Abstract

Bovine mastitis is characterized by inflammation of the mammary gland, usually due to bacterial infection, compromising quantity and quality of milk production. This study aimed to determine the antibacterial activity, in vitro, of the hydroalcoholic extract of the pomegranate peel at 10% against standard strains of bovine mastitis. The colonies were adjusted to the concentration of 107 ml-1 using UV-visible spectrophotometry, and the extracts were evaluated in quintuplicate in concentrations of 1000, 500, 250, 75, 50 and 25 μg ml-1. The sensitivity of the strains was determined using the minimum inhibitory concentration and disk diffusion test. Additionally, antioxidant activity and total phenolic content was evaluated. The extract, at concentrations of 500 and 1000 μg ml-1, inhibited Staphylococcus aureus (ATCC 25923), S. Saprophyticus (ATCC 15305), S. Epidermidis (ATCC 12228), Escherichia coli (ATCC 11229), Enterobacter cloacae (ATCC 23355 ) and Bacillus cereus (ATCC 33018), but it was not effective for Pseudomonas aeruginosa (ATCC 27853) and Salmonella enterica subspecie, enterica serovar Typhi (ATCC 19214). Antioxidant activity was observed from 50 μg ml-1 reaching a plateau at 500 mg ml-1 with 64.90%, and the concentration that causes 50% of the inhibition (IC50) corresponded to 378.80 μg l-1. Perhaps the presence of other substances in the extract may have been responsible for the antioxidant activity detected. That way, the antioxidant and antibacterial activities of EHPG 10% may represent an important therapeutic potential, particularly for animal health in organic and agroecological production systems.

 

Key words: Agroecology, medicinal plants, organic animal production, Punica granatum Linn.