Journal of
Medicinal Plants Research

  • Abbreviation: J. Med. Plants Res.
  • Language: English
  • ISSN: 1996-0875
  • DOI: 10.5897/JMPR
  • Start Year: 2007
  • Published Articles: 3674

Full Length Research Paper

DPPH radical scavenging activity of extracts from Urtica urens (Urticaceae)

Rets’epile Paul Matamane
  • Rets’epile Paul Matamane
  • 1Department of Chemistry and Chemical Technology, Faculty of Science and Technology, National University of Lesotho, Roma Campus, P. O. Roma 180, Kingdom of Lesotho, Southern Africa.
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Manoharan Karuppiah Pillai
  • Manoharan Karuppiah Pillai
  • 1Department of Chemistry and Chemical Technology, Faculty of Science and Technology, National University of Lesotho, Roma Campus, P. O. Roma 180, Kingdom of Lesotho, Southern Africa.
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Sibusisiwe Magama
  • Sibusisiwe Magama
  • 2Department of Biology, Faculty of Science and Technology, National University of Lesotho, Roma Campus, P. O. Roma 180, Kingdom of Lesotho, Southern Africa.
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  •  Received: 18 November 2019
  •  Accepted: 05 February 2020
  •  Published: 31 May 2020

Abstract

In this study, hexane, chloroform, ethyl acetate and methanolic extracts from leaves, stem-bark and root of Urtica urens were evaluated for their antioxidant activity by 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging assay. The hexane, chloroform, ethyl acetate and methanolic extracts from leaves of U. urens showed scavenging activity ranging from 7.06±2.52 to 26.01±1.84, 10.86±1.81 to 41.48±1.91, 7.53±3.21 to 51.85±4.20 and 51.50±1.97 to 73.84±6.82%,  respectively, at various concentrations. The hexane, chloroform, ethyl acetate and methanolic extracts from stem-bark of U. urens showed scavenging activity ranging from 3.26±1.84 to 38.54±2.78, 2.93±1.02 to 56.56±3.16, 19.19±1.77 to 53.99±2.18 and 30.10±0.07 to 62.80±1.90%, respectively, at various concentrations. The chloroform and methanolic extracts from root of U. urens showed scavenging activity ranging from 9.57±1.39 to 46.31±2.35 and 38.53±9.18 to 76.51±2.02%, respectively, at various concentrations. Additionally, the IC50 values of these extracts were also determined and was found to be in the range of <200 to >3000 µg/mL. The positive control, ascorbic acid, exhibited an IC50 value of <200 µg/mL. U. urensis reported to have many therapeutic applications, which include treating asthma, hearting related problems, pulmonary tuberculosis and cleansing the bladder. Basotho tribes use U. urens during spring season to increase iron content in the blood. Further studies on U. urens are required to explore this plant for its commercial applications.

Key words: Urtica urens, DPPH radical scavenging assay, ascorbic acid, solvent extracts.