Journal of
Medicinal Plants Research

  • Abbreviation: J. Med. Plants Res.
  • Language: English
  • ISSN: 1996-0875
  • DOI: 10.5897/JMPR
  • Start Year: 2007
  • Published Articles: 3778

Full Length Research Paper

Elicitation effect on production of plumbagin in in vitro culture of Drosera indica L.

Juengwatanatrakul Thaweesak1, Sakamoto Seiichi2, Tanaka Hiroyuki2 and Putalun Waraporn3*
1Faculty of Pharmaceutical Sciences, Ubon Ratchathani University, Ubon Ratchathani34190, Thailand. 2Graduate School of Pharmaceutical Sciences, Kyushu University, Fukuoka 812-8582, Japan. 3Faculty of Pharmaceutical Sciences, Khon Kaen University, Khon Kaen, 40002, Thailand.
Email: [email protected]

  •  Accepted: 29 July 2011
  •  Published: 23 September 2011

Abstract

Influence of elicitors on plumbagin accumulation in Drosera indica whole plant culture was investigated. Treatment of plantlets with 150 mg/l chitosan and 100 μM salicylic acid mostly stimulated plumbagin production after 3 days of elicitation, while 0.5 mg/ml yeast extract and 50 μM methyl jasmonate elicited at 6 days. Yeast extract (0.5 mg/ml) was the most efficient elicitor to enhance plumbagin production (2.69±0.03 mg/g dry wt.) by 5.4-fold over the control plant (0.50±0.01 mg/g dry wt.). Present results indicated the effectiveness of elicitation on plumbagin accumulation in in vitro culture of D. indica.

 

Key words: Drosera indica, plumbagin, elicitors.