Investigations have proved the presence of components such as cinamaldehyde, cinamyl acetate and phenolic compounds in Ocotea quixos, these components have been attributed to antimicrobial disinfectants, antifungal and healing activities. It is important to point out that, to evaluate any biological activity, it is necessary to have standardized extracts to obtain reliable and reproducible results. The present investigation consist of standardizer hydroalcoholic extracts of ishpingo, O. quixos, evaluating the influence of three experimental variables: vegetal material (fresh and dry), solvent (ethanol and cane ethanol) and solvent concentration (90, 70 and 50%) v/v through an experimental factorial design, from which 12 different extracts with 3 repetitions of each were obtained. The extracts were obtained through a percolation process considering the variables of the experimental design. A phytochemical screening showed the presence of polyphenols, tannins, catechins, saponins, quinones, coumarins, lactones and alkaloids in the 36 extracts; the Folin- Ciocalteu method was used in order to quantitatively determine the total phenol concentration and, by statistical analysis ANOVA (Tukey 95%), it was proved that there is a significant difference between the 12 established treatments where the extracts with the highest quantity of polyphenols are AMF50:50 (alcohol, fresh material, concentration 50% v/v) and CMF90:10 (cane alcohol, fresh material, concentration 90% v/v) with 12,538 and 13, 298 mg of gallic acid / mL of extract, respectively; concluding that, this last combination obtain greater quantity of total polyphenols following the established standardized protocol.
Key words: Ocotea quixos, ishpingo, total polyphenols, folin ciocalteu, phytochemical screening, gallic acid.
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