Journal of
Medicinal Plants Research

  • Abbreviation: J. Med. Plants Res.
  • Language: English
  • ISSN: 1996-0875
  • DOI: 10.5897/JMPR
  • Start Year: 2007
  • Published Articles: 3693

Full Length Research Paper

Phytochemical compounds and antibacterial activity of Jatropha curcas Linn. extracts

A. Namuli1, N. Abdullah2,3*, C. C. Sieo1,4, S. W. Zuhainis1 and E. Oskoueian1
1Department of Microbiology, Universiti Putra Malaysia, 43400 UPM, Serdang, Selangor, Malaysia. 2Department of Biochemistry, Universiti Putra Malaysia, 43400 UPM, Serdang, Selangor, Malaysia. 3Institute of Tropical Agriculture, Universiti Putra Malaysia, 43400 UPM, Serdang, Selangor, Malaysia. 4Institute of Bioscience, Universiti Putra Malaysia, 43400 UPM, Serdang, Selangor, Malaysia.
Email: [email protected]

  •  Accepted: 20 May 2011
  •  Published: 18 August 2011

Abstract

The present study was conducted to determine the phytochemical compounds in different solvent extracts of Jatropha curcas Linn. plant and antibacterial activity of crude extracts. Aqueous, methanolic and hexane extracts of various plant parts were analysed for phytochemical compounds by spectrophotometry, high performance liquid chromatography (HPLC) and gas chromatography-mass spectrometry analysis (GC-MS). Antibacterial activity was studied by paper disc diffusion assay against Gram positive and Gram negative bacteria. The minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) were determined by micro-broth dilution. The root bark methanolic extract contained high phenolics (11.51 mg gallic acid equivalents/g DW) and flavonoids (0.94 mg rutin equivalents/g DW). Kernel meal aqueous extract contained high saponins (0.65 mg diosgenin equivalents/g DW) and the methanolic extract contained 1.13 mg/g DW phorbol esters. Phytochemicals detected by RP-HPLC were pyrogallol, gallic acid, naringin, rutin and vanillic acid. The main compounds detected by GC-MS were oxalic acid (root bark), acetic acid and oleic acid (stem bark). Inhibition zones ranged from 8.0 to 17.7 mm. Low MIC (1.2 to 2.3 mg/ml) and MBC (0.4 to 6.3 mg/ml) values were observed in methanolic extract of all plant parts.  The present study showed that stem bark, root bark and kernel meal of J. curcas contained compounds with antibacterial activities. The results indicate the potential of J. curcas as a source of antibacterial compounds.

 

Key words: Jatropha curcas Linn., phorbol esters,  phytochemicals, antibacterial activity.