Journal of
Medicinal Plants Research

  • Abbreviation: J. Med. Plants Res.
  • Language: English
  • ISSN: 1996-0875
  • DOI: 10.5897/JMPR
  • Start Year: 2007
  • Published Articles: 3813

Full Length Research Paper

Quantification of α-, β- and γ-mangostin in Garcinia mangostana fruit rind extracts by a reverse phase high performance liquid chromatography

Abdalrahim F. A. Aisha1, Khalid M. Abu-Salah2, Mohammad J. Siddiqui3, Zhari Ismail1and Amin Malik Shah Abdul Majid1*
1School of Pharmaceutical Sciences, University of Science Malaysia (USM), Minden 11800, Pulau Pinang, Malaysia. 2Biochemistry Department and King Abdulla Institute for Nanotechnology, King Saud University (KSU), Riyadh 11451, Saudi Arabia. 3International Medical University (IMU), Kuala Lumpur 57000, Malaysia.
Email: [email protected]

  •  Accepted: 01 February 2012
  •  Published: 31 August 2012


Garcinia mangostana fruit rinds contain high concentration of xanthones such as α-, and γ-mangostin. The α-mangostin-rich extracts have been used widely in nutritional supplements, herbal cosmetics and pharmaceutical preparations. This study aims to develop a reverse phase HPLC method for the quantification of α-, β- and γ-mangostin in G. mangostana fruit rind extracts. The method was validated at 244, 254, 316 and 320 nm. Selectivity was determined by comparing the retention time and the UV-Vis spectra of α-, β- and γ-mangostin in G. mangostana extracts with those of the reference compounds. Linearity was in the range 0.2 to 200 µg/ml at R2 > 0.9999. The intraday and interday precision was determined as a relative standard deviation, and was found to be (0.4±0.4) and (0.3 ± 0.3)%, respectively. The percentage recovery was in the range (96.3 ± 2.5) to (100.5 ± 3.4)%. The limits of detection and quantification were in the range 0.06 to 0.12 and 0.14 to 0.37 µg/ml, respectively. The reported method was applied for the determination of α-, β- and γ-mangostin concentration in 7 extracts of G. mangostana fruit rinds, and it could be considered as an important analytical tool for quality control, stability studies, pharmacokinetics, and standardization purposes.


Key words: Alpha-, beta-, and gamma-mangostin, Garcinia mangostana, mangosteen.