Journal of
Medicinal Plants Research

  • Abbreviation: J. Med. Plants Res.
  • Language: English
  • ISSN: 1996-0875
  • DOI: 10.5897/JMPR
  • Start Year: 2007
  • Published Articles: 3843

Full Length Research Paper

Antibacterial potentials of Quercus baloot Griff.

Muhammad Khurram1,2*, Abdul Hameed2, Murad Ali Khan3, Muhammad Usman Amin4,  Muhammad Hassan5, Naseem Ullah1, Wajeeha Manzoor6, Abdul Qayum7, Muhammad Bilal8, Umberin Najeeb9 and Farhat Ali Khan1
1Department of Pharmacy, Faculty of Life Sciences, Sarhad University of Science and Information Technology, Peshawar, Pakistan. 2Department of Microbiology, Quaid-i-Azam University, Islamabad, Pakistan. 3Department of Chemistry, Kohat University of Science and Technology, Kohat, Pakistan. 4Department of Pharmacology, Kohat Institute of Medical Sciences, Kohat, Pakistan. 5Gandhara College of Pharmacy, Gandhara University, Peshawar, Pakistan. 6Frontier College for Women, Peshawar, Pakistan. 7Department of Pharmacology, Peshawar Medical College, Peshawar, Pakistan. 8Department of Plastic surgery, Khyber Teaching Hospital, Peshawar, Pakistan. 9Department of Medicine, University of Toronto, Toronto, Canada.
Email: pharmacistkhurram@hotmail

  •  Accepted: 27 December 2011
  •  Published: 23 February 2012

Abstract

Quercus baloot fractions were subjected to antibacterial susceptibility evaluation that illustrated inhibition zones against Staphylococcus aureus, Micrococcus luteus, Bacillus subtilis,Escherichia coli, and Pseudomonas aeruginosa. The active fractions were chromatographed by thin layer chromatography (TLC) and then contact bioautography was utilized as hyphenated bioassay, which showed inhibition zones at different Rf values against B. subtilis, M. luteus, E. coli, and S. aureus. The minimum inhibitory concentrations (MICs) of active fractions were found using a 96-well micro-titer plate method and the non-viability of the organisms was ascertained by determining the minimum bactericidal concentration (MBC) of the fractions. This is the first report of antibacterial activities for Q. baloot.

 

Key words: Quercus baloot, contact bioautography, minimum inhibitory concentration (MIC),minimum bactericidal concentration (MBC).

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This article is published under the terms of the Creative Commons Attribution License 4.0

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