Journal of
Medicinal Plants Research

  • Abbreviation: J. Med. Plants Res.
  • Language: English
  • ISSN: 1996-0875
  • DOI: 10.5897/JMPR
  • Start Year: 2007
  • Published Articles: 3835

Full Length Research Paper

Protective effect of puerarin form the roots of Pueraria lobata against systemic inflammatory response syndrome by regulating the levels of related cytokines

Sheng Quan1, Jun-bo Wang1, Xiao-ping Xia2, Xv-ai Lin3 and Jie Yan3*
1Department of Clinical Medicine, Zhejiang University City College, Hangzhou 310015, P. R. China. 2Clinical Laboratory of Sir Run Run Shaw Hospital, Zhejiang University, Hangzhou 310016, P. R. China. 3Department of Medical Microbiology and Parasitology, College of Medicine, Zhejiang University, Hangzhou 310058, P. R. China.
Email: [email protected]

  •  Accepted: 01 April 2011
  •  Published: 04 July 2011

Abstract

The outcome of systemic inflammatory response syndrome (SIRS) is poor due to unclear pathogenesis and unsatisfied therapeutic strategies. Therefore, to understand the pathogenetic mechanism and screen novel drugs are critical for the improvement of the therapeutic efficacy of SIRS. In the present study, to determine the protective mechanism of puerarin on SIRS, Sprague Dawley (SD) rats were intraperitoneally injected with different doses of zymosan-A to generate an experimental SIRS animal model. The peripheral TNF-α, IL-6 and IL-10 levels of SIRS rats were measured using quantitative ELISA assay. Protective effects puerarin on SIRS rats via regulating cytokine levels was subsequently determined. The results showed about 75.0% of SIRS rats died after injection with 1000 mg/kg zymosan-A, whereas only 16.7% of SIRS rats (1000 mg/kg zymosan-A) were found dead after the treatment with 62.5 mg/kg puerarin (P< 0.01 vs SIRS group). 24 h after injection with 750 mg/kg zymosan-A, the peripheral levels of TNF-α, IL-6 and IL-10 were 30.87±6.81 pg/ml, 525.20±92.45 pg/ml and 1.37±0.17 ng/ml, respectively. However, the levels of TNF-α and IL-6 (16.71±3.75 pg/ml and 399.30±77.87 pg/ml, respectively) were significantly lowered (P< 0.01) and IL-10 level (1.95±0.17 ng/ml) was markedly elevated (P< 0.01) after treatment with 62.5 mg/kg puerarin when compared with those in SIRS group. Puerarin conferred protective effects on the SIRS via down-regulating pro-inflammatory TNF-α and IL-6 as well as up-regulating anti-inflammatory IL-10.

 

Key words: Experimental SIRS, puerarin, protective effects, cytokines.