Journal of
Medicinal Plants Research

  • Abbreviation: J. Med. Plants Res.
  • Language: English
  • ISSN: 1996-0875
  • DOI: 10.5897/JMPR
  • Start Year: 2007
  • Published Articles: 3843

Full Length Research Paper

Cellular antioxidant and antiproliferative activity of ethanolic extract of Coleus tuberosus

Nugraheni, M.1*, Santoso, U.2, Suparmo2 and Wuryastuti, H.3
1Departement of Education of Food Engineering, Faculty of Engineering, Yogyakarta State University, Yogyakarta 55281, Indonesia. 2Department of Food and Agricultural Product Technology, Faculty of Agricultural Technology, Gadjah Mada University, Yogyakarta 55281, Indonesia. 3Departement of Internal Medicine, Veterinary Clinical Center, Faculty of Veterinary Medicine/Inter University Center for Food and Nutrition, Gadjah Mada University, Yogyakarta 55281, Indonesia.
Email: [email protected]

  •  Accepted: 09 May 2011
  •  Published: 31 August 2012

Abstract

Ethanolic extract of both the flesh and peel of Coleus tuberosus (EEPC) were evaluated for cellular antioxidant activity based on 2,7,-diacetate dichlorofluorescein (DCFH) oxidation, and antiproliferative activity based on 3-(4,5-dimethylthiazol-2)-2,5-diphenyltetrazolium bromide (MTT) assay, using human breast cancer MCF-7 cells. The result showed that antioxidant and antiproliferative activity of EEPC was higher than EEFC. Its bioactive compounds ursolic acid was higher than oleanolic acid. The antioxidant and antiproliferative activities were in a dose-dependent manner. The cellular antioxidant and antiproliferative activity of EEFC and EEPC were found related to oleanolic acid and ursolic acid content of both flesh and peel of C. tuberosus. These results indicated that EEFC and EEPC and its bioactive compounds reduced phorbol miristate acetate (PMA)-induce oxidative stress, might be used as a potential source of natural antioxidants and antiproliferative agent.

 

Key words: Antioxidant, antiproliferative, Coleus tuberosus; ursolic acid, oleanolic acid.

Abbreviation

EEFC, Ethanolic extract of flesh of Coleus tuberosus; EEPC, ethanolic extract of peel of Coleus tuberosus; MTT, 3-(4,5-dimethylthiazol-2)-2,5-diphenyltetrazolium bromide; DMEM, Dulbecco’s Modified Eagle Medium; FBS, fetal bovine serum; DCFH,2,,7,-diacetate dichlorofluorescein; PMA, Phorbol Miristate Acetate.

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This article is published under the terms of the Creative Commons Attribution License 4.0

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