Journal of
Medicinal Plants Research

  • Abbreviation: J. Med. Plants Res.
  • Language: English
  • ISSN: 1996-0875
  • DOI: 10.5897/JMPR
  • Start Year: 2007
  • Published Articles: 3596

Full Length Research Paper

Effect of hormones on the seed germination of Bupleurum species

Ma Yu
  • Ma Yu
  • Department of Agronomy, School of Life Science and Engineering, Southwest University of Science and Technology, 59 Qinglong Road, Mianyang, Sichuan 621010, PR China.
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Guihua Lv
  • Guihua Lv
  • Dongyang Institute of Maize Research, Zhejiang Academy of Agricultural Sciences, Dongyang, Zhejiang, 322100, China.
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Hongzhang
  • Hongzhang
  • Department of Agronomy, School of Life Science and Engineering, Southwest University of Science and Technology, 59 Qinglong Road, Mianyang, Sichuan 621010, PR China.
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Yuchan Li
  • Yuchan Li
  • Department of Agronomy, School of Life Science and Engineering, Southwest University of Science and Technology, 59 Qinglong Road, Mianyang, Sichuan 621010, PR China.
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Cheng Yuan
  • Cheng Yuan
  • Department of Agronomy, School of Life Science and Engineering, Southwest University of Science and Technology, 59 Qinglong Road, Mianyang, Sichuan 621010, PR China.
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Zhikang Tang
  • Zhikang Tang
  • Department of Agronomy, School of Life Science and Engineering, Southwest University of Science and Technology, 59 Qinglong Road, Mianyang, Sichuan 621010, PR China.
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Xiaoyan Shu
  • Xiaoyan Shu
  • Department of Agronomy, School of Life Science and Engineering, Southwest University of Science and Technology, 59 Qinglong Road, Mianyang, Sichuan 621010, PR China.
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Chun Sui
  • Chun Sui
  • Institute of Medicinal Plant Development (IMPLAD), Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing, 100193, China.
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Dabin Hou
  • Dabin Hou
  • Department of Agronomy, School of Life Science and Engineering, Southwest University of Science and Technology, 59 Qinglong Road, Mianyang, Sichuan 621010, PR China.
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Jian-He Wei
  • Jian-He Wei
  • Institute of Medicinal Plant Development (IMPLAD), Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing, 100193, China.
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  •  Received: 18 November 2018
  •  Accepted: 21 December 2018
  •  Published: 10 February 2019

Abstract

In Asia, Radix Bupleuri (Bupleurum spp. root) is an important medicine used for treating many diseases over the past 2000 years. However, its cultivation is difficult due to poor germination rates and loss of seedling viability shortly after seeding. In the present study, the effect of 6-benzyl aminopurine (6-BA), daminozide, gibberellin (GA3), paclobutrazol, uniconazole, and mepiquat chloride (MC) on the germination of seeds of ‘Zhongchai No. 3’, which is the newest released commercial variety of Bupleurum chinense DC in China was analyzed. Only GA3 was found to promote seed germination significantly, and its optimum concentration was 223.84 to 238.78 mg∙L-1. In addition, incubation with GA3 on the first day of germination was extremely important for seedling survival. This protocol could also be applied to ‘Zhonghongchai No. 1’ (B. scorzonerifolium Willd.) and ‘Hubei chaihu’ (B. chinense DC.). The combination of GA3 treatment and centralized incubation could be an efficient way to industrialize the production of secondary metabolites from Bupleurum species.

Key words: Hormone, Bupleurum spp., germination, gibberellin (GA3).