Journal of
Medicinal Plants Research

  • Abbreviation: J. Med. Plants Res.
  • Language: English
  • ISSN: 1996-0875
  • DOI: 10.5897/JMPR
  • Start Year: 2007
  • Published Articles: 3778

Full Length Research Paper

Cytotoxic flavonoids from Diplotaxis harra (Forssk.) Boiss. growing in Sinai

Magdy M. D. Mohammed1, 2*, Eman R. El-Sharkawy3 and Azza A. Matloub2    
1Institut für Umweltforschung (INFU), Technische Universität Dortmund, Otto-Hahn-Strasse 6, D-44221 Dortmund, Germany. 2Department of Pharmacognosy, National Research Center, Dokki-12311, Cairo, Egypt. 3Department of Ecology, Desert Research Center, Mathef El-Mataria, 15753, Egypt.    
Email: [email protected]

  •  Accepted: 22 June 2011
  •  Published: 03 January 2013

Abstract

As part of our ongoing collaborative effort to discover the anticancer activity of the phenolics isolated from terrestrial plant sources, the EtOH extract of the aerial parts of the Egyptian medicinal plant Diplotaxis harra (Forssk.) Boiss. was in vitro investigated for cytotoxicity against HCT116, HepG2 and MCF-7 cell lines, and resulted with IC50 = 4.65, 12.60 and 17.90 µg/ml respectively. Doxorubicin (+ve control) showed in vitro cytotoxic activity with IC50 = 3.64, 4.57 and 2.97 µg/ml respectively. The phenolic-rich fraction of the EtOH extract was subjected to further fractionation, which led to the isolation of five flavonoids; identified as quercetin, quercetin 3-O-β-glucoside, isorhamnetin 7-O-β-glucoside, apigenin 7-O-β-rhamnoside and kaempferol 3-O-β-glucoside, according to its’ spectral data and comparison with the literature. Furthermore, the isolated flavonoids showed in vitrocytotoxicity against HCT116 cell line with IC50 = 20.1, 24.3, 22.8, 23.4 and 41.9 µg/ml as determined by NADH DIAPHORASE (MTT method).

 

Keywords: Diplotaxis harra (Forssk.) Boiss., cruciferaeflavonoid glycosides, cytotoxicity.