Journal of
Medicinal Plants Research

  • Abbreviation: J. Med. Plants Res.
  • Language: English
  • ISSN: 1996-0875
  • DOI: 10.5897/JMPR
  • Start Year: 2007
  • Published Articles: 3813

Full Length Research Paper

Insecticidal properties of Citrus hystrix DC leaves essential oil against Spodoptera litura fabricius

Fan Siew Loh1, Rita Muhamad Awang1*, Dzolkhifli Omar1 and Mawardi Rahmani2
1Department of Plant Protection, Faculty of Agriculture, Universiti Putra Malaysia, 43400 UPM Serdang, Selangor, Malaysia. 2Department of Chemistry, Faculty of Science, Universiti Putra Malaysia, 43400 UPM Serdang, Selangor, Malaysia.
Email: [email protected]

  •  Accepted: 24 May 2011
  •  Published: 18 August 2011


Chemical analyisis by GC and GC-MS revealed presence of 29 compounds in the essential oil fraction of kaffir lime, Citrus hystrix fresh leaves. Beta-citronellal was the major compound present with 66.85% of total oil followed by beta-citronellol (6.59%), linalool (3.90%) and citronellol (1.76%). Insecticidal properties of C. hystrix leaves essential oil was investigated against tobacco army worm, Spodoptera litura using topical application bioassay on uniform weighted second instar larvae in the laboratory. Essential oil was effectively in killing the larvae and showed that the LD50 is 26.748 µL/g. Insect development and growth index observations showed that the essential oil had antifeedant properties resulting in severe growth inhibition of S. litura.


Key words: Citrus hystrixSpodoptera litura, essential oil, botanical insecticides.